Document Detail


Cell swelling-induced ATP release is tightly dependent on intracellular calcium elevations.
MedLine Citation:
PMID:  15579539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mechanical stresses release ATP from a variety of cells by a poorly defined mechanism(s). Using custom-designed flow-through chambers, we investigated the kinetics of cell swelling-induced ATP secretion, cell volume and intracellular calcium changes in epithelial A549 and 16HBE14o- cells, and NIH/3T3 fibroblasts. Fifty per cent hypotonic shock triggered transient ATP release from cell confluent monolayers, which consistently peaked at around 1 min 45 s for A549 and NIH/3T3, and at 3 min for 16HBE14o- cells, then declined to baseline within the next 15 min. Whereas the release time course had a similar pattern for the three cell types, the peak rates differed significantly (294 +/- 67, 70 +/- 22 and 17 +/- 2.8 pmol min(-1) (10(6) cells)(-1), for A549, 16HBE14o- and NIH/3T3, respectively). The concomitant volume changes of substrate-attached cells were analysed by a 3-dimensional cell shape reconstruction method based on images acquired from two perpendicular directions. The three cell types swelled at a similar rate, reaching maximal expansion in 1 min 45 s, but differed in the duration of the volume plateau and regulatory volume decrease (RVD). These experiments revealed that ATP release does not correlate with either cell volume expansion and the expected activation of stretch-sensitive channels, or with the activation of volume-sensitive, 5-nitro-2-(3-phenylpropylamino) benzoic acid-inhibitable anion channels during RVD. By contrast, ATP release was tightly synchronized, in all three cell types, with cytosolic calcium elevations. Furthermore, loading A549 cells with the calcium chelator BAPTA significantly diminished ATP release (71% inhibition of the peak rate), while the calcium ionophore ionomycin triggered ATP release in the absence of cell swelling. Lowering the temperature to 10 degrees C almost completely abolished A549 cell swelling-induced ATP release (95% inhibition of the peak rate). These results strongly suggest that calcium-dependent exocytosis plays a major role in mechanosensitive ATP release.
Authors:
Francis Boudreault; Ryszard Grygorczyk
Related Documents :
16663309 - Solutes contributing to osmotic adjustment in cultured plant cells adapted to water str...
12183519 - Human salivary histatin 5 causes disordered volume regulation and cell cycle arrest in ...
7733229 - Na-k-cl cotransport regulates intracellular volume and monolayer permeability of trabec...
3036129 - Evidence that the alpha and alpha (+)isoforms of the catalytic subunit of (na+, k+)-atp...
16754349 - Effects of bafilomycin a1, a vacuolar type h+ atpase inhibitor, on the thermosensitivit...
6885649 - The volume density of cells and capillaries of the normal stria vascularis.
19891779 - A selective eradication of human nonhereditary breast cancer cells by phenanthridine-de...
396649 - G cell distribution in normal duodenal mucosa.
20405239 - Protective effect of pacap against doxorubicin-induced cell death in cardiomyocyte cult...
Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-10-07
Journal Detail:
Title:  The Journal of physiology     Volume:  561     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-06     Completed Date:  2005-04-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  499-513     Citation Subset:  IM    
Affiliation:
Research Centre, CHUM-Hôtel-Dieu, 3850 Saint-Urbain, Montréal, Québec, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism,  secretion*
Animals
Calcium / metabolism*
Cell Line, Tumor
Cell Size*
Humans
Intracellular Fluid / metabolism,  secretion*
Mice
NIH 3T3 Cells
Chemical
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 7440-70-2/Calcium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The alpha 1 and alpha 6 subunit subtypes of the mammalian GABA(A) receptor confer distinct channel g...
Next Document:  Heteromeric KCNE2/KCNQ1 potassium channels in the luminal membrane of gastric parietal cells.