Document Detail


Cell survival within pulp and periodontal constructs.
MedLine Citation:
PMID:  19084127     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to measure cell survival and degradation within tissue-engineered dental constructs. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PLSCs) were seeded on three types of tissue engineering scaffolds: a synthetic open-cell D,D-L,L-polylactic acid (polymer) scaffold, a bovine collagen scaffold (collagen), and a calcium phosphate bioceramic (calcium phosphate) scaffold. The dental pulp and periodontal constructs (n = 144) were maintained in cell culture for between 3 and 14 days. The cell survival and degradation within the constructs were measured using histologic criteria. The DPSC and PLSC survival was optimal in the polymer and collagen constructs but not the calcium phosphate constructs, especially over longer time periods. These in vitro results suggest that both the polymer and collagen scaffolds and the DPSCs and PLSCs can be combined to create pulp and periodontal constructs for use in future regenerative dental treatments.
Authors:
Matthew Gebhardt; Peter E Murray; Kenneth N Namerow; Sergio Kuttler; Franklin Garcia-Godoy
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Publication Detail:
Type:  Journal Article     Date:  2008-11-07
Journal Detail:
Title:  Journal of endodontics     Volume:  35     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-03-09     Revised Date:  2013-04-05    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  63-6     Citation Subset:  D    
Affiliation:
Department of Endodontics, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL 33328-2018, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult Stem Cells / physiology*
Animals
Calcium Phosphates
Cattle
Cell Culture Techniques
Cell Survival
Cells, Cultured
Collagen
Dental Pulp / cytology*
Humans
L Cells (Cell Line)
Lactic Acid
Materials Testing
Mice
Periodontal Ligament / cytology*
Polymers
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
Chemical
Reg. No./Substance:
0/Calcium Phosphates; 0/Polymers; 26100-51-6/poly(lactic acid); 50-21-5/Lactic Acid; 9007-34-5/Collagen; 97Z1WI3NDX/calcium phosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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