Document Detail


Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform.
MedLine Citation:
PMID:  19015542     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proper assembly and maintenance of epithelia are critical for normal development and homeostasis. Here, using the Drosophila ovary as a model, we identify a role for the B1 isoform of the ecdysone receptor (EcR-B1) in this process. We performed a reverse genetic analysis of EcR-B1 function during oogenesis and demonstrate that silencing of this receptor isoform causes loss of integrity and multilayering of the follicular epithelium. We show that multilayered follicle cells lack proper cell polarity with altered distribution of apical and basolateral cell polarity markers including atypical-protein kinase C (aPKC), Discs-large (Dlg), and Scribble (Scrib) and aberrant accumulation of adherens junctions and F-actin cytoskeleton. We find that the EcR-B1 isoform is required for proper follicle cell polarity both during early stages of oogenesis, when follicle cells undergo the mitotic cell cycle, and at midoogenesis when these cells stop dividing and undergo several endocycles. In addition, we show that the EcR-B1 isoform is required during early oogenesis for follicle cell survival and that disruption of its function causes apoptotic cell death induced by caspase.
Authors:
Patrizia Romani; Fabio Bernardi; Jennifer Hackney; Leonard Dobens; Giuseppe Gargiulo; Valeria Cavaliere
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-11-17
Journal Detail:
Title:  Genetics     Volume:  181     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-13     Completed Date:  2009-03-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-75     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, University of Missouri, Kansas City, Missouri 64110, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Caspase 3 / metabolism
Cell Polarity*
Cell Survival
Clone Cells
Drosophila Proteins / metabolism
Drosophila melanogaster / cytology*,  enzymology
Enzyme Activation
Epithelium / metabolism
Female
Gene Knockdown Techniques
Gene Silencing
Inhibitor of Apoptosis Proteins / metabolism
Oogenesis
Ovarian Follicle / cytology*,  enzymology
Ovum / cytology,  metabolism
Protein Isoforms / deficiency,  metabolism
Receptors, Steroid / deficiency,  metabolism*
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Inhibitor of Apoptosis Proteins; 0/Protein Isoforms; 0/Receptors, Steroid; 0/ecdysone receptor; 0/thread protein, Drosophila; EC 3.4.22.-/Caspase 3
Comments/Corrections

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