Document Detail


Cell surface sialomucin and resistance to natural cell-mediated cytotoxicity of rat mammary tumor ascites cells.
MedLine Citation:
PMID:  3731108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MAT-B1 and MAT-C1 ascites sublines of the 13762 rat mammary adenocarcinoma both contain sialomucin as a major cell surface component and are resistant to cytolysis by normal rat spleen lymphocytes [3 +/- 2% (SD) and 0 +/- 1%, respectively]. Susceptibility to lysis did not increase following treatment of cells with neuraminidase, fucosidase, or alpha- or beta-galactosidase. Treatment with trypsin significantly increased the susceptibility of MAT-B1 (14 +/- 3%) but not MAT-C1 (5 +/- 2%). Following 1 month in culture, the sialomucin content of MAT-B1 cells dropped from 30% to 8% (determined by glucosamine labeling) and natural cell-mediated cytolysis increased to 16 +/- 4%, whereas the sialomucin content and susceptibility of MAT-C1 cells did not change. The results indicate that the relatively minor changes associated with removal of cell surface sialic acid or fucose residues do not result in increased susceptibility of the ascites cells to cytolysis. However, susceptibility of MAT-B1 cells to lysis by normal rat spleen lymphocytes was inversely correlated with the amount of major glycoprotein (r = -0.96).
Authors:
A P Sherblom; C E Moody
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  46     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1986-09-18     Completed Date:  1986-09-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4543-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascites / immunology*
Asialoglycoproteins / metabolism
Cytotoxicity, Immunologic*
Female
Galactosidases / metabolism
Glycoproteins / metabolism
Immunity, Innate
Killer Cells, Natural / immunology*
Mammary Neoplasms, Experimental / immunology*,  pathology
Neuraminidase / metabolism
Rats
Trypsin / metabolism
Grant Support
ID/Acronym/Agency:
CA33238/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Asialoglycoproteins; 0/Glycoproteins; EC 3.2.1.-/Galactosidases; EC 3.2.1.18/Neuraminidase; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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