| Cell surface and serum protein phosphorylation by U-937 cell ectoprotein kinases. | |
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MedLine Citation:
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PMID: 9063566 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Incubation of intact U-937 cells with 0.1 microM [gamma-32P]ATP for 10 min resulted in Mg(2+)-independent radiolabelling of about 40 cell surface proteins, some of which became more intensely labelled when cells were differentiated. Presence of 2% newborn calf serum revealed major labelling of three serum proteins with molecular weights of 170, 85 and 61 kDa. Out of several tested purified human serum proteins, complement factor 1s (C1s) exhibited specific labelling of the 28 kDa subunit. This capacity of monocytic cell ectokinases to phosphorylate cell surface and serum proteins may have significance for the regulation of interactions between these cells and their environment. In addition, phosphorylation of components of the complement system suggest a possible new means of regulating the immune response through the action of extracellular kinases. |
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Authors:
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T Geberhiwot; G Skoglund |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry and molecular biology international Volume: 41 ISSN: 1039-9712 ISO Abbreviation: Biochem. Mol. Biol. Int. Publication Date: 1997 Feb |
Date Detail:
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Created Date: 1997-06-11 Completed Date: 1997-06-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9306673 Medline TA: Biochem Mol Biol Int Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 269-78 Citation Subset: IM |
Affiliation:
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Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood Proteins
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metabolism* Cell Differentiation Cell Line Complement C1s / metabolism* Electrophoresis, Polyacrylamide Gel Humans Membrane Proteins / metabolism* Monocytes / enzymology* Phosphorus Radioisotopes Phosphorylation Protein Kinases / physiology* Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins; 0/Membrane Proteins; 0/Phosphorus Radioisotopes; EC 2.7.-/Protein Kinases; EC 2.7.1.-/ectoprotein kinase; EC 3.4.21.42/Complement C1s |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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