| Cell surface oxygen consumption: a major contributor to cellular oxygen consumption in glycolytic cancer cell lines. | |
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MedLine Citation:
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PMID: 17266920 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxygen consumption for bioenergetic purposes has long been thought to be the prerogative of mitochondria. Nevertheless, mitochondrial gene knockout (rho(0)) cells that are defective in mitochondrial respiration require oxygen for growth and consume oxygen at the cell surface via trans-plasma membrane electron transport (tPMET). This raises the possibility that cell surface oxygen consumption may support glycolytic energy metabolism by reoxidising cytosolic NADH to facilitate continued glycolysis. In this paper we determined the extent of cell surface oxygen consumption in a panel of 19 cancer cell lines. Non-mitochondrial (myxothiazol-resistant) oxygen consumption was demonstrated to consist of at least two components, cell surface oxygen consumption (inhibited by extracellular NADH) and basal oxygen consumption (insensitive to both myxothiazol and NADH). The extent of cell surface oxygen consumption varied considerably between parental cell lines from 1% to 80% of total oxygen consumption rates. In addition, cell surface oxygen consumption was found to be associated with low levels of superoxide production and to contribute significantly (up to 25%) to extracellular acidification in HL60rho(0) cells. In summary, cell surface oxygen consumption contributes significantly to total cellular oxygen consumption, not only in rho(0) cells but also in mitochondrially competent tumour cell lines with glycolytic metabolism. |
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Authors:
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Patries M Herst; Michael V Berridge |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-06 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1767 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-02-27 Completed Date: 2007-04-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 170-7 Citation Subset: IM |
Affiliation:
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Malaghan Institute of Medical Research, P.O. Box 7060, Wellington, New Zealand. pherst@malaghan.org.nz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / pharmacology Cell Line, Tumor Cell Membrane / metabolism* Culture Media Glycolysis / physiology* HL-60 Cells Hela Cells Humans Hydrogen Peroxide / metabolism Hydrogen-Ion Concentration Methacrylates / pharmacology Methylphenazonium Methosulfate / analogs & derivatives, metabolism Mice NAD / pharmacology Oxygen Consumption / physiology* Superoxides / metabolism Tetrazolium Salts / metabolism Thiazoles / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; 0/Culture Media; 0/Methacrylates; 0/Tetrazolium Salts; 0/Thiazoles; 11062-77-4/Superoxides; 299-11-6/Methylphenazonium Methosulfate; 370-86-5/Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; 53-84-9/NAD; 65162-13-2/1-methoxy-5-methylphenazinium methyl sulfate; 76706-55-3/myxothiazol; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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