Document Detail

Cell surface glycosaminoglycans are not involved in the adherence of Helicobacter pylori to cultured Hs 198.St human gastric cells, Hs 746T human gastric adenocarcinoma cells, or HeLa cells.
MedLine Citation:
PMID:  8910015     Owner:  NLM     Status:  MEDLINE    
Hs 198.St cells (a line derived from normal human gastric tissue), Hs 746T cells (a line derived from human gastric adenocarcinoma), and HeLa cells were used together with 3H-labelled Helicobacter pylori, strain NCTC 11637 to determine if cell surface glycosaminoglycans could act as initial receptors for adherence of the bacteria. Although as much as 40% of the 3H-labelled bacteria adhered to monolayers of the cultured cells, removal of glycosaminoglycans by prior treatment of the cells with heparitinase, heparinase, or chondroitin ABC lyase had no effect in modifying the adherence. Prior addition of heparan sulfate, heparin, or chondroitin/dermatan sulfate to bacteria had no effect on adherence, nor were bacteria released when these same glycosaminoglycans or these same enzymes were added to cultures already containing adherent bacteria. These results indicated that neither heparan sulfate nor chondroitin/dermatan sulfate are involved as receptors in the initial adherence step of H. pylori to these cultured cells.
H Kawasaki; G Sugumaran; J E Silbert
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  13     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-02-13     Completed Date:  1997-02-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  873-7     Citation Subset:  IM    
Edith Nourse Rogers Memorial Veterans Hospital, Bedford, MA 01730, USA.
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MeSH Terms
Bacterial Adhesion / physiology*
Cell Line
Chondroitin Lyases / metabolism
Glycosaminoglycans / metabolism,  pharmacology
Hela Cells
Helicobacter pylori / metabolism*
Heparin Lyase
Membrane Glycoproteins / metabolism*
Polysaccharide-Lyases / metabolism
Receptors, Cell Surface / chemistry,  metabolism
Trypsin / metabolism
Tumor Cells, Cultured
Reg. No./Substance:
0/Glycosaminoglycans; 0/Membrane Glycoproteins; 0/Receptors, Cell Surface; EC; EC 4.2.2.-/Chondroitin Lyases; EC 4.2.2.-/Polysaccharide-Lyases; EC Lyase; EC lyase

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