| Cell-surface-expressed T-cell antigen-receptor zeta chain is associated with the cytoskeleton. | |
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MedLine Citation:
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PMID: 7761399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The T-cell antigen receptor zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. zeta chain can associate with certain protein tyrosine kinases and retains the capacity to transduce signals independently of the other receptor subunits. Thus, zeta chain could couple cell-surface-expressed T-cell antigen receptors to the intracellular signal-transduction apparatus by its association with various intracellular molecules in addition to tyrosine kinases. In the process of searching for zeta chain-associated molecules we observed that after lysis of resting T cells with Triton X-100, zeta chain is localized in the detergent-insoluble fraction, in addition to its presence in the detergent-soluble fraction. Treatment of T cells with cytochalasin B, an actin-depolymerizing agent, leads to the complete dissociation of zeta chain from the Triton-insoluble fraction, suggesting a linkage between zeta chain and the cytoskeletal matrix. We have also determined that cytoskeletal-associated zeta chain is expressed on the cell surface. Furthermore, a tyrosine-phosphorylated 16-kDa zeta chain was detected only in the Triton-insoluble cytoskeletal fraction of resting T cells. zeta chain also maintains its association with the cytoskeleton when expressed in COS cells, inferring that the cytoskeletal elements involved in this linkage may be ubiquitous. Finally, we have localized a 42-amino acid region in the intracytoplasmic domain of zeta chain, which is crucial for maximal interaction between zeta chain and the cytoskeleton. Anchorage of cell-surface-expressed zeta chain to the cytoskeleton in resting T cells may facilitate recycling of receptor complexes and/or allow the transduction of external stimuli into the cell. |
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Authors:
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S Caplan; S Zeliger; L Wang; M Baniyash |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 92 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-06-29 Completed Date: 1995-06-29 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4768-72 Citation Subset: IM |
Affiliation:
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Lautenberg Center for General and Tumor Immunology, Hebrew University-Hadassah Medical School, Jerusalem, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Cell Line Cell Membrane / immunology Cells, Cultured Cercopithecus aethiops Cytochalasin B / pharmacology Cytoskeleton / drug effects, immunology* Female Hybridomas / immunology Membrane Proteins / analysis, biosynthesis* Mice Mice, Inbred BALB C Molecular Sequence Data Receptors, Antigen, T-Cell / analysis, biosynthesis* Recombinant Proteins / analysis, biosynthesis Spleen / immunology T-Lymphocytes / drug effects, immunology* Thymus Gland / immunology Transfection |
| Chemical | |
Reg. No./Substance:
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0/Membrane Proteins; 0/Receptors, Antigen, T-Cell; 0/Recombinant Proteins; 0/antigen T cell receptor, zeta chain; 14930-96-2/Cytochalasin B |
| Comments/Corrections | |
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