Document Detail


Cell-surface-bound Yersinia translocate the protein tyrosine phosphatase YopH by a polarized mechanism into the target cell.
MedLine Citation:
PMID:  8596454     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
YopH is translocated by cell-surface-bound bacteria through the plasma membrane to the cytosol of the HeLa cell. The transfer mechanism is contact dependent and polarizes the translocation to only occur at the contact zone between the bacterium and the target cell. More than 99% of the PTPase activity is associated with the HeLa cells. In contrast to the wild-type strain, the yopBD mutant cannot deliver YopH to the cytosol. Instead YopH is deposited in localized areas in the proximity of cell-associated bacteria. A yopN mutant secretes 40% of the total amount of YopH to the culture medium, suggesting a critical role of YopN in regulation of the polarized translocation. Evidence for a region in YopH important for its translocation through the plasma membrane of the target cell but not for secretion from the pathogen is provided.
Authors:
C Persson; R Nordfelth; A Holmström; S Håkansson; R Rosqvist; H Wolf-Watz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  18     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-04-17     Completed Date:  1996-04-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  135-50     Citation Subset:  IM    
Affiliation:
Department of Cell and Molecular Biology, Umeå University, Sweden.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U18727
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Adhesion*
Bacterial Outer Membrane Proteins / genetics,  immunology,  secretion*
Base Sequence
Biological Transport
Cell Membrane / metabolism
Cell Polarity*
Fluorescent Antibody Technique
Hela Cells
Humans
Macrophages
Mice
Molecular Chaperones / genetics
Molecular Sequence Data
Mutation
Phagocytosis
Protein Tyrosine Phosphatases / immunology,  secretion*
Yersinia pseudotuberculosis / metabolism*
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Molecular Chaperones; 0/SycH protein, Yersinia; 0/YopB protein, Yersinia; 0/YopD protein, Yersinia; EC 3.1.3.48/Protein Tyrosine Phosphatases; EC 3.1.3.48/yopH protein, Yersinia

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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