Document Detail


Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles.
MedLine Citation:
PMID:  22521612     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Optimal cell therapies require efficient, selective and rapid delivery of molecular cargo into target cells without compromising their viability. Achieving these goals ex vivo in bulk heterogeneous multi-cell systems such as human grafts is impeded by low selectivity and speed of cargo delivery and by significant damage to target and non-target cells. We have developed a cell level approach for selective and guided transmembrane injection of extracellular cargo into specific target cells using transient plasmonic nanobubbles (PNB) as cell-specific nano-injectors. As a technical platform for this method we developed a laser flow cell processing system. The PNB injection method and flow system were tested in heterogeneous cell suspensions of target and non-target cells for delivery of Dextran-FITC dye into squamous cell carcinoma HN31 cells and transfection of human T-cells with a green fluorescent protein-encoding plasmid. In both models the method demonstrated single cell type selectivity, high efficacy of delivery (96% both for HN31 cells T-cells), speed of delivery (nanoseconds) and viability of treated target cells (96% for HN31 cells and 75% for T-cells). The PNB injection method may therefore be beneficial for real time processing of human grafts without removal of physiologically important cells.
Authors:
Ekaterina Y Lukianova-Hleb; Daniel S Wagner; Malcolm K Brenner; Dmitri O Lapotko
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-04-20
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-05-18     Completed Date:  2012-09-17     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  5441-50     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD3 / metabolism
Cell Adhesion
Cell Line, Tumor
Cell Membrane / metabolism*
Extracellular Space / metabolism
Gold / chemistry*
Humans
Injections
Metal Nanoparticles / chemistry*
Nanotechnology / methods*
Organ Specificity
Suspensions
T-Lymphocytes / metabolism
Transfection
Grant Support
ID/Acronym/Agency:
P50 CA126752/CA/NCI NIH HHS; R01 GM094816/GM/NIGMS NIH HHS; R01GM094816/GM/NIGMS NIH HHS; S10 RR026399/RR/NCRR NIH HHS; S10RR026399-01/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD3; 0/Suspensions; 7440-57-5/Gold
Comments/Corrections

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