| Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles. | |
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MedLine Citation:
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PMID: 22521612 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Optimal cell therapies require efficient, selective and rapid delivery of molecular cargo into target cells without compromising their viability. Achieving these goals ex vivo in bulk heterogeneous multi-cell systems such as human grafts is impeded by low selectivity and speed of cargo delivery and by significant damage to target and non-target cells. We have developed a cell level approach for selective and guided transmembrane injection of extracellular cargo into specific target cells using transient plasmonic nanobubbles (PNB) as cell-specific nano-injectors. As a technical platform for this method we developed a laser flow cell processing system. The PNB injection method and flow system were tested in heterogeneous cell suspensions of target and non-target cells for delivery of Dextran-FITC dye into squamous cell carcinoma HN31 cells and transfection of human T-cells with a green fluorescent protein-encoding plasmid. In both models the method demonstrated single cell type selectivity, high efficacy of delivery (96% both for HN31 cells T-cells), speed of delivery (nanoseconds) and viability of treated target cells (96% for HN31 cells and 75% for T-cells). The PNB injection method may therefore be beneficial for real time processing of human grafts without removal of physiologically important cells. |
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Authors:
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Ekaterina Y Lukianova-Hleb; Daniel S Wagner; Malcolm K Brenner; Dmitri O Lapotko |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-04-20 |
Journal Detail:
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Title: Biomaterials Volume: 33 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-05-18 Completed Date: 2012-09-17 Revised Date: 2012-12-25 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 5441-50 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD3
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metabolism Cell Adhesion Cell Line, Tumor Cell Membrane / metabolism* Extracellular Space / metabolism Gold / chemistry* Humans Injections Metal Nanoparticles / chemistry* Nanotechnology / methods* Organ Specificity Suspensions T-Lymphocytes / metabolism Transfection |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM094816/GM/NIGMS NIH HHS; R01GM094816/GM/NIGMS NIH HHS; S10 RR026399/RR/NCRR NIH HHS; S10RR026399-01/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD3; 0/Suspensions; 7440-57-5/Gold |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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