Document Detail


Cell-specific translational regulation of S-adenosylmethionine decarboxylase mRNA. Influence of the structure of the 5' transcript leader on regulation by the upstream open reading frame.
MedLine Citation:
PMID:  8027046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A small upstream open reading frame (uORF), located 14 nucleotides from the cap in the 5' transcript leader (5' TL) of the mRNA encoding S-adenosylmethionine decarboxylase (AdoMetDC), suppresses translation of the downstream cistron in normal T cells and T cell lines. In the present study, we examined the structural features of the 5' TL that overcome this suppressive influence in cells of nonlymphoid origin. Initiation at the downstream cistron in nonlymphoid cells is by a cap-dependent mechanism that requires ribosome scanning along the 5' TL and does not involve an internal ribosome entry site. Extending the uORF so that it overlapped the major cistron by 101 nucleotides had no effect on translation of the downstream cistron in either HeLa or Jurkat cells. When the distance between the uORF and the cap was extended to 47 nucleotides, using sequence previously found to be neutral, translation of the major cistron was inhibited 5-fold in HeLa cells and the mRNA was moved from polysomes to monosomes, a location identical to that of the wild type mRNA in Jurkat cells. Therefore, in contrast to T cells, initiation at the uORF seems to be relatively infrequent in non-lymphoid cells due to its proximity to the cap, allowing efficient translation of the downstream cistron.
Authors:
H Ruan; J R Hill; S Fatemie-Nainie; D R Morris
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  269     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-08-08     Completed Date:  1994-08-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17905-10     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Washington, Seattle 98195.
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MeSH Terms
Descriptor/Qualifier:
Adenosylmethionine Decarboxylase / genetics*
Base Sequence
Cell Line
Cloning, Molecular
DNA
Gene Expression Regulation*
Hela Cells
Humans
Molecular Sequence Data
Open Reading Frames
Protein Biosynthesis
RNA, Messenger / genetics*
T-Lymphocytes / cytology
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
CA39053/CA/NCI NIH HHS; GM07266/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 9007-49-2/DNA; EC 4.1.1.50/Adenosylmethionine Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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