| Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions. | |
| | |
MedLine Citation:
|
PMID: 22240379 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Acetylcholine (ACh) has been shown to exert an anti-inflammatory function by down-modulating the expression of pro-inflammatory cytokines. Its availability can be regulated at different levels, namely at its synthesis and degradation steps. Accordingly, the expression of acetylcholinesterase (AChE), the enzyme responsible for ACh hydrolysis, has been observed to be modulated in inflammation. To further address the mechanisms underlying this effect, we aimed here at characterizing AChE expression in distinct cellular types pivotal to the inflammatory response. This study was performed in the human acute leukaemia monocytyc cell line, THP-1, in human monocyte-derived primary macrophages and in human umbilical cord vein endothelial cells (HUVEC). In order to subject these cells to inflammatory conditions, THP-1 and macrophage were treated with lipopolysaccharide (LPS) from E.coli and HUVEC were stimulated with the tumour necrosis factor α (TNF-α). Our results showed that although AChE expression was generally up-regulated at the mRNA level under inflammatory conditions, distinct AChE protein expression profiles were surprisingly observed among the distinct cellular types studied. Altogether, these results argue for the existence of cell specific mechanisms that regulate the expression of acetylcholinesterase in inflammation. |
| | |
Authors:
|
P de Oliveira; A Q Gomes; T R Pacheco; V Vitorino de Almeida; C Saldanha; A Calado |
Related Documents
:
|
9707479 - In vitro application of testosterone potentiates gonadotropin-releasing hormone-stimula... 22427949 - Perilipin 2 (plin2)-deficiency does not increase cholesterol-induced toxicity in macrop... 21826199 - Anti-inflammatory effect of fluvastatin on il-8 production induced by pseudomonas aerug... 20837449 - Basophils: new players in the cytokine network. 14627199 - Antigenic peptides complexed to phylogenically diverse hsp70s induce differential immun... 17344089 - Modulation of immunological synapse by membrane-bound and soluble ligands. 22120429 - Vitamin a and immune regulation: role of retinoic acid in gut-associated dendritic cell... 12887599 - The allograft inflammatory factor-1 in creutzfeldt-jakob disease brains. 15710649 - The complement inhibitory protein daf (cd55) suppresses t cell immunity in vivo. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-3 |
Journal Detail:
|
Title: Clinical hemorheology and microcirculation Volume: - ISSN: 1875-8622 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9709206 Medline TA: Clin Hemorheol Microcirc Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of cigarette smoking on morphology and aggregation of erythrocytes.
Next Document: Erythrocyte as a biological sensor.