| Cell-specific in vivo DNA-protein interactions at the proximal promoters of the pro alpha 1(I) and the pro alpha2(I) collagen genes. | |
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MedLine Citation:
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PMID: 9241239 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We performed in vivo dimethylsulfate footprinting of the 220 bp mouse proximal proalpha1(I) collagen promoter and the 350 bp mouse proximal proalpha2(I) collagen promoter in BALB/3T3 fibroblasts, primary mouse skin fibroblasts, S-194 B cells, NMuLi liver epithelial cells and RAG renal adenocarcinoma cells and in vitro DNase I footprinting of these promoters using nuclear extracts of these different cell types. Whereas proalpha1(I) and proalpha2(I) collagen RNAs were present in BALB/3T3 fibroblasts and primary fibroblasts, these RNAs could not be detected in the three other cell lines. Comparison of in vitro DNase I footprints for each of the two proximal collagen promoters indicated that the patterns of protection were very similar with the different nuclear extracts, suggesting that the DNA binding proteins binding to these promoters were present in all cell types tested. In contrast, in vivo footprints over these proximal promoters were cell-specific, occurring only in fibroblast cells and not in the other three cell types. The in vivo footprints were generally located within the in vitro footprinted regions. Our results suggest that although all cell types tested contained nuclear proteins that can bind to the proximal proalpha1(I) and proalpha2(I) collagen promoters in vitro , it is only in fibroblasts that these proteins bind to their cognate sites in vivo . We discuss possible regulatory mechanisms in type I collagen genes that can contribute to the cell-specific in vivo protein-DNA interactions at the proximal promoters. |
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Authors:
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S S Chen; E C Ruteshouser; S N Maity; B de Crombrugghe |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleic acids research Volume: 25 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1997 Aug |
Date Detail:
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Created Date: 1997-09-17 Completed Date: 1997-09-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3261-8 Citation Subset: IM |
Affiliation:
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Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Base Sequence Cell Line Cell Nucleus / physiology Collagen / genetics* DNA Footprinting DNA-Binding Proteins / chemistry* Deoxyribonuclease I / diagnostic use Fibroblasts / physiology Liver / physiology Mice Molecular Sequence Data Procollagen / genetics* Promoter Regions, Genetic* Sulfuric Acid Esters / chemistry |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Procollagen; 0/Sulfuric Acid Esters; 77-78-1/dimethyl sulfate; 9007-34-5/Collagen; EC 3.1.21.1/Deoxyribonuclease I |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 15 Month: 8 Year: 1997 Volume: 25 Issue: 16 First Page: 3261 Last Page: 3268 ID: 146878 PubMed Id: 9241239 Publisher Item Identifier: gka534 |
| Cell-specific in vivo DNA-protein interactions at the proximal promoters of the pro alpha 1(I) and the pro alpha2(I) collagen genes. | |
| S S Chen | |
| E C Ruteshouser | |
| S N Maity | |
| B de Crombrugghe | |
| Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA. |
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