Document Detail

Cell-specific expression of betaC-activin in the rat reproductive tract, adrenal and liver.
MedLine Citation:
PMID:  15249126     Owner:  NLM     Status:  MEDLINE    
betaC-activin expression was assessed in rat tissues, using reverse transcription and real-time polymerase chain reaction, Western blotting and immunohistochemistry with a specific monoclonal antibody. betaC-activin mRNA was predominantly expressed in liver, but significant amounts were found in rat whole pituitary extracts (n = 5), and in three of five extracts of ovary, testis, and adrenal gland. Specific betaC-activin immunoreactivity was demonstrated in the cytoplasm of hepatocytes, neurosecretory cell terminals in posterior pituitary, ovarian primordial follicles, theca interna, large luteal cells and rete ovarii, spermatogonia, pachytene spermatocytes and Leydig cells of the testis, uterine endometrium, oviduct epithelium and zona glomerulosa of the adrenal. The observation of stage-specific expression in gonadal cells suggests this activin subunit has specific roles, different from those of other activin/inhibin subunits. Small amounts of mRNA in the presence of significant betaC-activin protein highlights the importance of examining betaC-activin expression at both the mRNA and protein level.
Elspeth J Gold; Moira K O'Bryan; Sally L Mellor; Mark Cranfield; Gail P Risbridger; Nigel P Groome; Jean S Fleming
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  222     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-13     Completed Date:  2005-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  61-9     Citation Subset:  IM    
Department of Anatomy and Structural Biology and Centre for Gene Research, University of Otago, School of Medical Sciences, P.O. Box 913, Dunedin, New Zealand.
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MeSH Terms
Adrenal Glands / metabolism*
Blotting, Western
Immunoenzyme Techniques
Inhibin-beta Subunits / genetics,  metabolism*
Liver / metabolism*
Ovary / metabolism*
RNA, Messenger / genetics,  metabolism
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Testis / metabolism*
Reg. No./Substance:
0/Inhbc protein, rat; 0/RNA, Messenger; 93443-12-0/Inhibin-beta Subunits

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