Document Detail


Cell-specific cytotoxicity of dextran-stabilized magnetite nanoparticles.
MedLine Citation:
PMID:  20427159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytotoxicity of dextran-hybridized magnetite nanoparticles which were prepared by a novel polyol method was evaluated by incubation with four different kinds of cells, including rat liver cells BRL 3A, renal cells NRK, astrocyte and periphery blood mononuclear cells (PBMC). The study was designed not only to evaluate their cytotoxicity but also to reflect the interaction between nanoparticles and related cells in their circulation processes. By fluorescent-activated cell sorting technique, it was found that the cytotoxicity of the nanoparticles is cell-specific. Under the concentrations in our study (0-128 mg/mL), the nanoparticles lead to the apoptosis of PBMC in a concentration-dependant manner, but have almost no influence on the other kinds of cells. TEM images demonstrate that the nanoparticles were endocytosed by BRL 3A, NRK and astrocyte, and result in the apoptosis of PBMC without the observation of the uptaking process. The results suggest that the related cells in nanoparticles cycling process should also be concerned for the cytotoxicity evaluation.
Authors:
Jing Ding; Ke Tao; Jiyu Li; Sheng Song; Kang Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-08
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  79     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  184-90     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Astrocytes / cytology,  drug effects
Cell Line
Cells, Cultured
Dextrans / chemistry*
Dose-Response Relationship, Drug
Ferrosoferric Oxide / chemistry,  toxicity*
Flow Cytometry
Leukocytes, Mononuclear / cytology,  drug effects
Metal Nanoparticles / chemistry,  toxicity,  ultrastructure
Microscopy, Electron, Transmission
Rats
Rats, Sprague-Dawley
X-Ray Diffraction
Chemical
Reg. No./Substance:
1317-61-9/Ferrosoferric Oxide; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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