Document Detail

Cell-specific activation of aflatoxin B1 correlates with presence of some cytochrome P450 enzymes in olfactory and respiratory tissues in horse.
MedLine Citation:
PMID:  12726741     Owner:  NLM     Status:  MEDLINE    
Horses may be exposed to aflatoxin B(1) (AFB(1)) via inhalation of mouldy dust, leading to high exposure of olfactory and respiratory tissues. In the present study the metabolic activation of AFB(1) was examined in olfactory and respiratory tissues in horse. The results showed covalent binding of AFB(1)-metabolites in sustentacular cells and cells of Bowman's glands in the olfactory mucosa, in some cells of the surface epithelium of nasal respiratory, tracheal, bronchial and bronchiolar mucosa and in some glands in these areas. Immunohistochemistry revealed that cells expressing proteins reacting with CYP 3A4- and CYP 2A6/2B6-antibodies had a similar distribution as those having capacity to activate AFB(1). Our data indicate that the cell-specific activation of AFB(1) correlates with presence of some CYP-enzymes in olfactory and respiratory tissues in horse.
P Larsson; E Persson; E Tydén; H Tjälve
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Research in veterinary science     Volume:  74     ISSN:  0034-5288     ISO Abbreviation:  Res. Vet. Sci.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-02     Completed Date:  2003-07-30     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  0401300     Medline TA:  Res Vet Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  227-33     Citation Subset:  IM    
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MeSH Terms
Aflatoxin B1 / pharmacokinetics*
Aryl Hydrocarbon Hydroxylases / metabolism
Cytochrome P-450 CYP3A
Cytochrome P-450 Enzyme System / metabolism
Enzyme Inhibitors / pharmacology
Flavonoids / pharmacology
Glutathione / metabolism
Horse Diseases / enzymology,  metabolism*
Immunohistochemistry / veterinary
Liver / enzymology
Lung / enzymology
Metyrapone / pharmacology
Microsomes / enzymology
Mixed Function Oxygenases / metabolism
Olfactory Mucosa / enzymology*
Respiratory Mucosa / enzymology*
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Flavanones; 0/Flavonoids; 9035-51-2/Cytochrome P-450 Enzyme System; 9N2N2Y55MH/Aflatoxin B1; EC 1.-/Mixed Function Oxygenases; EC 1.14.13.-/coumarin 7-hydroxylase; EC Hydrocarbon Hydroxylases; EC protein, human; EC P-450 CYP3A; GAN16C9B8O/Glutathione; HN5425SBF2/naringenin; ZS9KD92H6V/Metyrapone

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