Document Detail

Cell size specific binding of the fluorescent dye calcofluor to budding yeast.
MedLine Citation:
PMID:  2203478     Owner:  NLM     Status:  MEDLINE    
The yeast Saccharomyces cerevisiae cell surface outside of the bud scars displayed an increasing fluorescence intensity with increasing cell size (volume), where fluorescence was due to irreversible binding of the fluorescent dye calcofluor. The increase in fluorescence intensity appeared to be due to an increase in the density of fluorescence per unit surface area of the cell. Exposure time measurements from a photomicroscope were used to quantitate fluorescence intensity on individual cells. The cell size dependent increase in fluorescence intensity was displayed by unbudded cells from stationary phase populations, and unbudded and parent cells from exponentially growing populations. Abnormally large cells generated during the arrest of cell division with alpha-factor or restrictive temperature for cdc3, 8, 13, 24, and 28 cell division cycle mutants, displayed significantly greater fluorescence intensity compared to the smaller cells generated during the arrest of division for cdc25, 33, and 35 mutant strains. Fluorescence intensity on newly emerging buds was broadly dependent on both the size of the bud, and the size of the parent cells on which the buds were growing.
S A Moore
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1035     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-10-11     Completed Date:  1990-10-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  206-13     Citation Subset:  IM    
Department of Chemistry & Biochemistry California State University, Fullerton.
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MeSH Terms
Benzenesulfonates / metabolism*
Binding Sites
Cell Division
Cell Membrane / metabolism
Fluorescent Dyes
Peptides / pharmacology
Pheromones / pharmacology
Saccharomyces cerevisiae / cytology*,  genetics,  growth & development
Species Specificity
Spectrometry, Fluorescence
Reg. No./Substance:
0/Benzenesulfonates; 0/Fluorescent Dyes; 0/Peptides; 0/Pheromones; 4404-43-7/C.I. Fluorescent Brightening Agent 28; 61194-02-3/mating factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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