Document Detail

Cell size regulation in mammalian cells.
MedLine Citation:
PMID:  17245129     Owner:  NLM     Status:  MEDLINE    
The regulation of cell growth and proliferation is fundamental for animal development and homeostasis but the mechanisms that coordinate cell growth with cell cycle progression are poorly understood. One possibility is that "cell-size checkpoints" act to delay division until cells have achieved a minimal size or mass however, the existence of such checkpoints in mammalian cells is controversial. In this study we provide further evidence against the operation of a size checkpoint in mammalian cells. We show that primary mammalian cells proliferate at a rate that is independent of cell size or cell mass and that cell size is "set" by the balance of extracellular growth factors and mitogens. Moreover, we show that commonly used culture conditions stimulate cell growth much more than cell cycle progression resulting in cells that proliferate at sizes 300-500% larger than their in vivo counterparts. This has profound effects on cell behaviour.
Pedro Echave; Ian J Conlon; Alison C Lloyd
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-24
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  6     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-03-01     Completed Date:  2007-04-09     Revised Date:  2007-06-28    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  218-24     Citation Subset:  IM    
MRC Laboratory for Molecular Cell Biology and Department of Biochemistry, University College London, London, UK.
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MeSH Terms
Cell Culture Techniques / methods
Cell Proliferation
Cell Size*
Cells, Cultured
Fibroblasts / cytology*,  physiology
Schwann Cells / cytology*,  physiology

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