Document Detail

Cell size-proliferation relationship in rat glioma cells.
MedLine Citation:
PMID:  14730698     Owner:  NLM     Status:  MEDLINE    
The homeostasis of the central nervous system is highly controlled by glial cells and is dramatically altered in the case of glioma. In this respect, the complex connection between cell size and division is of particular importance and needs clarifying. In order to investigate this connection, cell number and volume were measured in C6 rat glioma cells under different experimental conditions, including continuous cell culture, Cl- channel blockade, and anisotonicity, and in the presence of an inhibitory conditioned medium collected from cell cultures or in a medium containing a low level of fetal calf serum. The rate of cell proliferation changed with cell volume in a bell-shaped manner, so that it is optimal within a cell volume window and appears to be controlled by low and high cell size checkpoints. The cell size-proliferation relationship can be defined by Boltzmann-like equations, which may reflect the effects of macromolecular crowding on proteins controlling the cell cycle progression. Altogether, these observations indicate that glioma cell proliferation is controlled predominantly but not exclusively by cell size-dependent mechanisms.
Béatrice Rouzaire-Dubois; Michel Malo; Jean-Baptiste Milandri; Jean-Marc Dubois
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glia     Volume:  45     ISSN:  0894-1491     ISO Abbreviation:  Glia     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-19     Completed Date:  2004-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-57     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Gif-sur-Yvette, France.
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MeSH Terms
Cell Count / methods
Cell Cycle / physiology*
Cell Death / physiology
Cell Division / physiology
Cell Line, Tumor
Cell Size / physiology
Glioma / pathology*

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