Document Detail


Cell size monitored counterflow centrifugation of human bone marrow resulting in clonogenic cell fractions substantially depleted of small lymphocytes.
MedLine Citation:
PMID:  6655238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human bone marrow cells were fractionated by physical methods in order to obtain cell fractions enriched in clonogenic cells and devoid of immunocompetent lymphocytes. The bulk of the erythrocytes was removed by isopycnic gradient centrifugation on Ficoll-Isopaque (d = 1.085 g/ml) and the majority of mature granulocytes on Percoll (d = 1.070 g/ml). The nucleated cells were separated into fractions by counterflow centrifugation. Continuous monitoring of the effluent of the elutriator by a light scatter device improved the reproducibility of the separation profiles. Progenitor cells did not form a single distinct peak and the maximal enrichment factor was 8.5. Lymphocytes were eliminated almost completely from the progenitor cell rich fraction (both CFU-GM and BFU-E). Physical elimination of lymphocytes from human bone marrow may offer an alternative approach to the prevention of graft-versus-host disease in allogeneic bone marrow transplantation.
Authors:
T De Witte; A Plas; E Koekman; G Blankenborg; M Salden; J Wessels; C Haanen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunological methods     Volume:  65     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  171-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigens, Surface / immunology
Bone Marrow / immunology
Bone Marrow Cells*
Cell Separation / methods*
Centrifugation / methods
Clone Cells / cytology,  immunology
Colony-Forming Units Assay
Erythrocytes / cytology
Hematopoietic Stem Cells / cytology,  immunology
Humans
Lymphocyte Depletion*
Lymphocytes* / cytology,  immunology
Chemical
Reg. No./Substance:
0/Antigens, Surface

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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