| Cell size monitored counterflow centrifugation of human bone marrow resulting in clonogenic cell fractions substantially depleted of small lymphocytes. | |
MedLine Citation:
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PMID: 6655238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human bone marrow cells were fractionated by physical methods in order to obtain cell fractions enriched in clonogenic cells and devoid of immunocompetent lymphocytes. The bulk of the erythrocytes was removed by isopycnic gradient centrifugation on Ficoll-Isopaque (d = 1.085 g/ml) and the majority of mature granulocytes on Percoll (d = 1.070 g/ml). The nucleated cells were separated into fractions by counterflow centrifugation. Continuous monitoring of the effluent of the elutriator by a light scatter device improved the reproducibility of the separation profiles. Progenitor cells did not form a single distinct peak and the maximal enrichment factor was 8.5. Lymphocytes were eliminated almost completely from the progenitor cell rich fraction (both CFU-GM and BFU-E). Physical elimination of lymphocytes from human bone marrow may offer an alternative approach to the prevention of graft-versus-host disease in allogeneic bone marrow transplantation. |
Authors:
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T De Witte; A Plas; E Koekman; G Blankenborg; M Salden; J Wessels; C Haanen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunological methods Volume: 65 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1983 Dec |
Date Detail:
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Created Date: 1984-02-14 Completed Date: 1984-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 171-82 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Surface
/
immunology Bone Marrow / immunology Bone Marrow Cells* Cell Separation / methods* Centrifugation / methods Clone Cells / cytology, immunology Colony-Forming Units Assay Erythrocytes / cytology Hematopoietic Stem Cells / cytology, immunology Humans Lymphocyte Depletion* Lymphocytes* / cytology, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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