| Cell shape and substrate rigidity both regulate cell stiffness. | |
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MedLine Citation:
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PMID: 21354386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells from many different tissues sense the stiffness and spatial patterning of their microenvironment to modulate their shape and cortical stiffness. It is currently unknown how substrate stiffness, cell shape, and cell stiffness modulate or interact with one another. Here, we use microcontact printing and microfabricated arrays of elastomeric posts to independently and simultaneously control cell shape and substrate stiffness. Our experiments show that cell cortical stiffness increases as a function of both substrate stiffness and spread area. For soft substrates, the influence of substrate stiffness on cell cortical stiffness is more prominent than that of cell shape, since increasing adherent area does not lead to cell stiffening. On the other hand, for cells constrained to a small area, cell shape effects are more dominant than substrate stiffness, since increasing substrate stiffness no longer affects cell stiffness. These results suggest that cell size and substrate stiffness can interact in a complex fashion to either enhance or antagonize each other's effect on cell morphology and mechanics. |
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Authors:
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Shang-You Tee; Jianping Fu; Christopher S Chen; Paul A Janmey |
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Publication Detail:
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Type: Journal Article; Research Support, American Recovery and Reinvestment Act; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biophysical journal Volume: 100 ISSN: 1542-0086 ISO Abbreviation: Biophys. J. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-28 Completed Date: 2011-06-02 Revised Date: 2012-03-06 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: L25-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biomechanics Cell Shape* Humans Mechanical Processes* Mesenchymal Stem Cells / cytology*, metabolism* Myosins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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EB00262/EB/NIBIB NIH HHS; GM083272-02S1/GM/NIGMS NIH HHS; HL90747/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.6.4.1/Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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