Document Detail

Cell shape and phenotypic expression in chondrocytes.
MedLine Citation:
PMID:  6828458     Owner:  NLM     Status:  MEDLINE    
The relationship between cell shape, proliferation, and phenotypic expression was studied in human chondrocytes. Shape was controlled independent of serum concentration, anchorage, and cell density by alteration of substratum adhesiveness with poly(2-hydroxyethyl methacrylate) (poly[HEMA]). Cells that were held rounded displayed features of the chondrocyte phenotype; i.e., they were round, proliferated slowly, incorporated low levels of [3H]thymidine into DNA, and incorporated large amounts of 35SO4 into glycosaminoglycans. In contrast, cells that were held flat were fibroblast-like: they exhibited flattened morphology, more rapid growth, greater incorporation of [3H]thymidine, and less incorporation of 35SO4. These studies suggest that cell shape may play an important role in phenotypic expression in chondrocytes.
J Glowacki; E Trepman; J Folkman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)     Volume:  172     ISSN:  0037-9727     ISO Abbreviation:  Proc. Soc. Exp. Biol. Med.     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-04-07     Completed Date:  1983-04-07     Revised Date:  2007-11-02    
Medline Journal Info:
Nlm Unique ID:  7505892     Medline TA:  Proc Soc Exp Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  93-8     Citation Subset:  IM    
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MeSH Terms
Cartilage / cytology,  physiology*
Cell Adhesion
Cells, Cultured
DNA Replication
Fibroblasts / physiology
Polyhydroxyethyl Methacrylate
Reg. No./Substance:
25249-16-5/Polyhydroxyethyl Methacrylate

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