Document Detail

Cell shape normalization, dendrite orientation, and melanin production of normal and genetically altered (haploinsufficient NF1)-melanocytes by microstructured substrate interactions.
MedLine Citation:
PMID:  14999847     Owner:  NLM     Status:  MEDLINE    
Little is known about how functional regulation failure in genetically altered cells is influenced by topographical confinement of cells, a situation often present in tissues in vivo. We used cultured melanocytes derived from human skin samples as a model system for such investigations. Normal melanocytes have a very well defined shape consisting of a cell body and two dendrites arranged 180 degrees relative to each other. In contrast, neurofibromin 1-melanocytes (NF1-melanocytes) have up to a 50% reduction of neurofibromin 1, which results in an altered morphology that can be easily measured. NF1-melanocytes deviate from the defined structure of normal melanocytes by forming more than two dendrites per cell. We show that morphology consequences of genetically altered melanocytes can be canceled if cells interact with substrates microstructured by stripes that apply mechanophysical signals in the form of physical topography. The strength of the mechanophysical signal was varied systematically by increasing the height of the microstructures. Melanocytes respond to surface topographical features that are larger than 50 nm and have lateral confinements smaller 4 microns. The response of normal and NF1-melanocytes to different topographies was analyzed quantitatively by determining density distributions for the number of dendrites per cell, the angles between dendrites, and the orientation imprinted in the substrate. The synthesis of melanin, a pigment produced by melanocytes, differs in the case of genetically altered NF1- and normal melanocytes. In both cases, the interaction with microstripes enhanced melanin production significantly. This enhanced melanin production is speculated to be caused by the mechanical stabilization of the dendrites by substrate guidance.
Simon Jungbauer; Ralf Kemkemer; Hans Gruler; Dieter Kaufmann; Joachim P Spatz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemphyschem : a European journal of chemical physics and physical chemistry     Volume:  5     ISSN:  1439-4235     ISO Abbreviation:  Chemphyschem     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-03-05     Completed Date:  2004-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100954211     Medline TA:  Chemphyschem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  85-92     Citation Subset:  IM    
University of Heidelberg, Biophysical Chemistry, Institute for Physical Chemistry, Im Neuenheimerfeld 253 69120 Heidelberg, Germany.
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MeSH Terms
Cell Size
Cells, Cultured
Culture Media
Dendrites / physiology*,  ultrastructure
Melanins / analysis,  biosynthesis*
Melanocytes / cytology,  metabolism,  physiology*
Microscopy, Electron
Neurofibromin 1 / chemistry,  genetics*
Skin Pigmentation
Reg. No./Substance:
0/Culture Media; 0/Melanins; 0/Neurofibromin 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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