Document Detail


Cell separation on microfabricated electrodes using dielectrophoretic/gravitational field-flow fractionation.
MedLine Citation:
PMID:  10079757     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dielectrophoretic/gravitational field-flow fractionation (DEP/G-FFF) was used to separate cultured human breast cancer MDA-435 cells from normal blood cells mixed together in a sucrose/dextrose medium. An array of microfabricated, interdigitated electrodes of 50 microns widths and spacings, and lining the bottom surface of a thin chamber (0.42 mm H x 25 mm W x 300 mm L), was used to generate DEP forces that levitated the cells. A 10-microL cell mixture sample containing approximately 50,000 cells was introduced into the chamber, and cancerous and normal blood cells were levitated to different heights according to the balance of DEP and gravitational forces. The cells at different heights were transported at different velocities under the influence of a parabolic flow profile that was established in the chamber and were thereby separated. Separation performance depended on the frequency and voltage of the applied DEP field and the fluid-flow rate. It took as little as 5 min to achieve cell separation. An analysis of the DEP/G-FFF results revealed that the separation exploited the difference in dielectric and density properties between cell populations. The DEP/G-FFF technique is potentially applicable to many biological and biomedical problems, especially those related to microfluidic systems.
Authors:
J Yang; Y Huang; X B Wang; F F Becker; P R Gascoyne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  71     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-20     Completed Date:  1999-04-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  911-8     Citation Subset:  IM    
Affiliation:
Department of Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Breast Neoplasms / pathology
Cell Separation / instrumentation*
Chemical Fractionation
Electrophysiology
Humans
Microelectrodes*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
R01 DK51065-01/DK/NIDDK NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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