Document Detail


Cell-selective labeling of bacterial proteomes with an orthogonal phenylalanine amino acid reporter.
MedLine Citation:
PMID:  22080199     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Orthogonal amino acid reporters allow the selective labeling of different cell types in heterogeneous populations through the expression of engineered aminoacyl tRNA synthetases. Here, we demonstrate that para-ethynylphenylalanine (PEP) can be used as an orthogonal amino acid reporter for efficient selective labeling of an intracellular bacterial pathogen during infection.
Authors:
Markus Grammel; Paul D Dossa; Emma Taylor-Salmon; Howard C Hang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-11
Journal Detail:
Title:  Chemical communications (Cambridge, England)     Volume:  -     ISSN:  1364-548X     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610838     Medline TA:  Chem Commun (Camb)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
The Rockefeller University, Laboratory of Chemical Biology and Microbial Pathogenesis, New York, USA. hhang@rockefeller.edu.
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