Document Detail

Cell seeding into calcium phosphate cement.
MedLine Citation:
PMID:  14986318     Owner:  NLM     Status:  MEDLINE    
To improve the effectiveness of calcium phosphate cement (CPC), we have developed a method to seed osteoblasts into the cement. CPC powder is mixed with water to form a paste that can be shaped to fit a bone defect in situ. The paste hardens in 30 min, reacts to form hydroxyapatite, and is replaced with new bone. Reacted CPC is biocompatible but unreacted CPC paste was found to have toxic effects when placed on cell monolayers (MC3T3-E1 cells). In contrast, when cells were indirectly exposed to CPC paste using a porous membrane or by placing a coverslip containing adherent cells onto a bed of CPC paste, the unreacted CPC was nontoxic. These results suggested that gel encapsulation of the cells might protect them from the CPC paste. Thus, cells were encapsulated in alginate beads (3.6-mm diameter), mixed with CPC paste, and incubated overnight. Both vital staining (calcein-AM and ethidium homodimer-1) and the Wst-1 assay (measures dehydrogenase activity) showed that cell survival in alginate beads that were mixed with CPC was similar to survival in untreated control beads. These results suggest that gel encapsulation could be used as a mechanism to protect cells for seeding into CPC.
Carl G Simon; William F Guthrie; Francis W Wang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  68     ISSN:  1549-3296     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-26     Completed Date:  2004-10-07     Revised Date:  2013-04-05    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  628-39     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Wiley Periodicals, Inc. J Biomed Mater Res 68A: 628-639, 2004
Polymers Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899-8545, USA.
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MeSH Terms
Bone Substitutes*
Calcium Phosphates*
Glucuronic Acid*
Hexuronic Acids*
Osteoblasts / physiology*
Silicone Oils
Grant Support
Reg. No./Substance:
0/Alginates; 0/Bone Substitutes; 0/Calcium Phosphates; 0/Hexuronic Acids; 0/Silicone Oils; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid; 97Z1WI3NDX/calcium phosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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