Document Detail

Cell replication and aging: in vitro and in vivo studies.
MedLine Citation:
PMID:  428565     Owner:  NLM     Status:  MEDLINE    
The relationship between human aging and cell replication has been investigated using two complementary approaches: in vitro studies of human fibroblasts derived from young and old volunteer members of the Baltimore Longitudinal Study and in vivo examinations of bone marrow cell populations from young and old mice and rats. Total proliferative capacity measured as either the onset of cell culture senescence or as in vitro life span was significantly diminished in cell cultures derived from old human donors when compared to parallel cultures established from young donors. Acute replicative abilities as measured by percent replicating cells, cell pupulation doubling time, cell number at confluency, and colony size distribution were also significantly decreased in human old cell populations. An in vivo cytogenetic technique for measuring cell replication was developed utilizing the differential staining properties of metaphase chromosomes of cells that have replicated in the presence of bromodeoxyuridine. With this technique, cell cycle times have been derived in vivo as well as in vitro. Preliminary in vivo results in both mice and rats indicate that cell replication is slowed in old animal cell populations. Further research will be directed both in vitro and in vivo at discerning the mechanisms for this impairment of cellular replication with aging.
E L Schneider
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Federation proceedings     Volume:  38     ISSN:  0014-9446     ISO Abbreviation:  Fed. Proc.     Publication Date:  1979 Apr 
Date Detail:
Created Date:  1979-06-29     Completed Date:  1979-06-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372771     Medline TA:  Fed Proc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1857-61     Citation Subset:  IM    
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MeSH Terms
Bone Marrow Cells
Cell Division*
Cell Survival*
Cells, Cultured / physiology
Chromatids / metabolism
Culture Media
DNA Replication
Skin / cytology
Time Factors
Reg. No./Substance:
0/Culture Media; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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