Document Detail

Cell- and region-specific expression of sugar chains in the mouse epididymal epithelium using lectin histochemistry combined with immunohistochemistry.
MedLine Citation:
PMID:  22645905     Owner:  NLM     Status:  In-Process    
To understand the cytochemical properties of epididymal epithelial cells, the characteristics of glycoconjugates in the mouse epididymis were examined using the technique of lectin histochemistry combined with immunohistochemistry. Characteristic staining patterns depending on the type of lectins were observed in the epididymal epithelium. Principal cells expressed N-acetyl-D-galactosamine (GalNAc), N-acetyl-D-glucosamine (GlcNAc), and Fucose in the proximal region of the epididymis and Mannose, Glucose, and Galactose in the distal region of the epididymis. Basal cells expressed Mannose, Glucose, Galactose, and GlcNAc in the proximal region and Galactose in the distal region. On the other hand, clear cells expressed various sugar residues and differences among regions were not observed. Interestingly, principal cells, clear cells, and basal cells specifically reacted with Ulex Europaeus-Agglutinin I (UEA-I lectin), Maackia Amurensis-Lectin I (MAL-I lectin), and Griffonia simplicifolia Lectin I-B4 (GS-I B lectin), respectively. These findings indicate that the selectivity in lectin reactivity for distinct cell types and segment-dependent staining in the epididymis may be related to cellular and regional differences in function. Furthermore, because some lectins stain particular cells or cellular compartments selectively, these lectins could be useful markers for histopathological evaluation of diseases or diagnosis of male infertility.
Shota Tajiri; Tatsuya Fukui; Akira Sawaguchi; Kazuya Yoshinaga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Okajimas folia anatomica Japonica     Volume:  88     ISSN:  0030-154X     ISO Abbreviation:  Okajimas Folia Anat Jpn     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-05-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401014     Medline TA:  Okajimas Folia Anat Jpn     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  141-6     Citation Subset:  IM    
Department of Anatomy and Cell Biology, Graduate School of Health Sciences, Kumamoto University, 4-24-1 Kuhonji, Kumamoto 862-0975, Japan.
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