Document Detail


Cell proteomic footprint.
MedLine Citation:
PMID:  19184978     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The authentication of mammalian cell cultures and their subpopulations is of great demand in biotechnology and cell therapy. However, current techniques are either not efficient or can be very complex and expensive. Here we report a simple and straightforward approach for authentication of biological cells and their subpopulations with high speed, high throughput, low sample cost, and high sensitivity. We discovered that cell cultures treated with protease under soft, 'non-killing' conditions release fragments of cell surface proteins, whose composition is a strong characteristic of the cells. Mass spectrometric analysis of the released fragments allows a direct comparison of the produced mass spectrum with the mass spectrum of known cells. As an example, we applied this technique to verify subpopulations of human fibroblasts with different origins and which exhibit different medical characteristics.
Authors:
Petr Lokhov; Elena Balashova; Maxim Dashtiev
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  23     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-12     Completed Date:  2009-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  680-2     Citation Subset:  IM    
Copyright Information:
John Wiley & Sons, Ltd
Affiliation:
Analytical Department, ZAO BioBohemia, Russia, Garibaldi st. 4, 117418 Moscow, Russia. lokhovpg@rambler.ru
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MeSH Terms
Descriptor/Qualifier:
Algorithms*
Biological Assay / methods
Cell Fractionation / methods*
Cells, Cultured
Fibroblasts / cytology*,  metabolism*
Gene Expression Profiling / methods*
Humans
Proteome / analysis,  chemistry*
Spectrometry, Mass, Electrospray Ionization / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Chemical
Reg. No./Substance:
0/Proteome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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