| Cell proteomic footprint. | |
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MedLine Citation:
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PMID: 19184978 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The authentication of mammalian cell cultures and their subpopulations is of great demand in biotechnology and cell therapy. However, current techniques are either not efficient or can be very complex and expensive. Here we report a simple and straightforward approach for authentication of biological cells and their subpopulations with high speed, high throughput, low sample cost, and high sensitivity. We discovered that cell cultures treated with protease under soft, 'non-killing' conditions release fragments of cell surface proteins, whose composition is a strong characteristic of the cells. Mass spectrometric analysis of the released fragments allows a direct comparison of the produced mass spectrum with the mass spectrum of known cells. As an example, we applied this technique to verify subpopulations of human fibroblasts with different origins and which exhibit different medical characteristics. |
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Authors:
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Petr Lokhov; Elena Balashova; Maxim Dashtiev |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 23 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-02-12 Completed Date: 2009-04-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 680-2 Citation Subset: IM |
Copyright Information:
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John Wiley & Sons, Ltd |
Affiliation:
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Analytical Department, ZAO BioBohemia, Russia, Garibaldi st. 4, 117418 Moscow, Russia. lokhovpg@rambler.ru |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms* Biological Assay / methods Cell Fractionation / methods* Cells, Cultured Fibroblasts / cytology*, metabolism* Gene Expression Profiling / methods* Humans Proteome / analysis, chemistry* Spectrometry, Mass, Electrospray Ionization / methods* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Proteome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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