| Cell proliferation and oxidative stress pathways are modified in fibroblasts from Sturge-Weber syndrome patients. | |
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MedLine Citation:
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PMID: 22402795 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sturge-Weber syndrome (SWS) is defined by vascular malformations of the face, eye and brain and an underlying somatic mutation has been hypothesized. We employed isobaric tags for relative and absolute quantification (iTRAQ-8plex)-based liquid chromatography interfaced with tandem mass spectrometry (LC-MS/MS) approach to identify differentially expressed proteins between port-wine-derived and normal skin-derived fibroblasts of four individuals with SWS. Proteins were identified that were significantly up- or down-regulated (i.e., ratios >1.2 or <0.8) in two or three pairs of samples (n = 31/972 quantified proteins) and their associated p values reported. Ingenuity pathway analysis (IPA) tool showed that the up-regulated proteins were associated with pathways that enhance cell proliferation; down-regulated proteins were associated with suppression of cell proliferation. The significant toxicologic list pathway in all four observations was oxidative stress mediated by Nrf2. This proteomics study highlights oxidative stress also consistent with a possible mutation in the RASA1 gene or pathway in SWS. |
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Authors:
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Shilpa D Kadam; Marjan Gucek; Robert N Cole; Paul A Watkins; Anne M Comi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-03-10 |
Journal Detail:
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Title: Archives of dermatological research Volume: 304 ISSN: 1432-069X ISO Abbreviation: Arch. Dermatol. Res. Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-03-21 Completed Date: 2012-07-20 Revised Date: 2013-05-14 |
Medline Journal Info:
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Nlm Unique ID: 8000462 Medline TA: Arch Dermatol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 229-35 Citation Subset: IM |
Affiliation:
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Departments of Neurology and Developmental Medicine, Hugo Moser Kennedy Krieger Research Institute, Baltimore, MD, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Cell Proliferation* Child Child, Preschool Chromatography, Liquid / methods Down-Regulation Female Fibroblasts / metabolism, pathology Humans Male NF-E2-Related Factor 2 / metabolism Oxidative Stress* Proteomics / methods Sturge-Weber Syndrome / metabolism*, pathology* Tandem Mass Spectrometry / methods Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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P30 DK089502/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/NF-E2-Related Factor 2; 0/NFE2L2 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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