Document Detail


Cell proliferation and oxidative stress pathways are modified in fibroblasts from Sturge-Weber syndrome patients.
MedLine Citation:
PMID:  22402795     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sturge-Weber syndrome (SWS) is defined by vascular malformations of the face, eye and brain and an underlying somatic mutation has been hypothesized. We employed isobaric tags for relative and absolute quantification (iTRAQ-8plex)-based liquid chromatography interfaced with tandem mass spectrometry (LC-MS/MS) approach to identify differentially expressed proteins between port-wine-derived and normal skin-derived fibroblasts of four individuals with SWS. Proteins were identified that were significantly up- or down-regulated (i.e., ratios >1.2 or <0.8) in two or three pairs of samples (n = 31/972 quantified proteins) and their associated p values reported. Ingenuity pathway analysis (IPA) tool showed that the up-regulated proteins were associated with pathways that enhance cell proliferation; down-regulated proteins were associated with suppression of cell proliferation. The significant toxicologic list pathway in all four observations was oxidative stress mediated by Nrf2. This proteomics study highlights oxidative stress also consistent with a possible mutation in the RASA1 gene or pathway in SWS.
Authors:
Shilpa D Kadam; Marjan Gucek; Robert N Cole; Paul A Watkins; Anne M Comi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-10
Journal Detail:
Title:  Archives of dermatological research     Volume:  304     ISSN:  1432-069X     ISO Abbreviation:  Arch. Dermatol. Res.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-21     Completed Date:  2012-07-20     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  8000462     Medline TA:  Arch Dermatol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  229-35     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Cell Proliferation*
Child
Child, Preschool
Chromatography, Liquid / methods
Down-Regulation
Female
Fibroblasts / metabolism,  pathology
Humans
Male
NF-E2-Related Factor 2 / metabolism
Oxidative Stress*
Proteomics / methods
Sturge-Weber Syndrome / metabolism*,  pathology*
Tandem Mass Spectrometry / methods
Up-Regulation
Chemical
Reg. No./Substance:
0/NF-E2-Related Factor 2; 0/NFE2L2 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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