Document Detail


Cell proliferation in ventromedial hypothalamic lesioned rats inhibits acute gastric mucosal lesions.
MedLine Citation:
PMID:  24331526     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
AIM: The role of mucosal layer thickness on prevention of acute gastric mucosal lesions (AGMLs) was examined in ventromedial hypothalamic (VMH)-lesioned rats.
MATERIALS AND METHODS: The incidence of AGMLs after 48-h fasting and 60% ethanol injection into the stomach after 24-h fasting, aggressive factors (gastric acid and serum gastrin) and defensive factors [hexosamine, gastric mucosal blood flow (GMBF), serum thiobarbituric acid reacting substances (TBARS), and thickness of the gastric mucosal layer] were evaluated in VMH-lesioned rats. The effects of cell proliferation on the gastric mucosal layer of these rats were evaluated by H-E staining and immunostaining with proliferating cell nuclear antigen (PCNA).
RESULTS: After 48-h fasting, no AGMLs were observed in VMH-lesioned and sham VMH-lesioned rats (controls). With 60% ethanol administration after 24-h fasting, the numbers of AGMLs were similar in the two groups, but the ulcer index, a marker of ulcer formation, was lower in VMH-lesioned rats compared to that in sham VMH-lesioned rats. VMH-lesioned rats showed increased gastric acid secretion and serum gastrin compared to sham VMH-lesioned rats, indicating an increase in aggressive factors in VMH-lesioned rats. The two groups had similar levels of gastric mucosal hexosamine, GMBF, and gastric mucosal TBARS, but VMH-lesioned rats had an increased thickness of the mucosal cell layer, indicating an increase in defensive factors in these rats. Histologically, VMH-lesioned rats had an increased total mucosal cell layer, especially for the surface epithelial cell layer, and an increased PCNA-labeling index, a marker of cell proliferation, especially in the proliferative zones of gastric mucosa, indicating increased cell proliferation in the proliferative zone of the gastric mucosa.
CONCLUSION: VMH-lesioned rats are resistant to AGML formation due to increased cell proliferation in gastric mucosa through elevating the levels of defensive factors over those of aggressive factors.
Authors:
Noriko Ishizuka; Nobuo Imazeki; Akira Senoo; Junko Sakurai; Masaru Sonoda; Masao Kanazawa; Yoko Suzuki; Yoko Kobayashi; Tosei Takahashi; Ryota Haba; Katsumi Arai; Hiroyuki Shimizu; Kahoru Sasaki; Masako Kako; Kaori Hayashi; Yuichi Suzuki; Shuji Inoue
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Obesity research & clinical practice     Volume:  6     ISSN:  1871-403X     ISO Abbreviation:  Obes Res Clin Pract     Publication Date:    2012 Jul-Sep
Date Detail:
Created Date:  2013-12-16     Completed Date:  2013-12-17     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  101303911     Medline TA:  Obes Res Clin Pract     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  e175-262     Citation Subset:  -    
Copyright Information:
© 2012 Published by Elsevier Ltd on behalf of Asia Oceania Assoc. for the Study of Obesity.
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