Document Detail

Cell proliferation in normal and diseased gastric mucosa. Autoradiography after in vitro continuous labelling with tritiated thymidine.
MedLine Citation:
PMID:  463568     Owner:  NLM     Status:  MEDLINE    
The rate of gastric epithelial cell proliferation was studied in healthy volunteers and in patients with different degrees of gastritis. Endoscopic biopsies from the antral and fundic part of the stomach were incubated in vitro with 3H-thymidine for 30, 120, and 210 minutes respectively. Autoradiographs were prepared, and the percentage of DNA-synthesizing cells (labeling index) in the progenitor cell region was estimated. From the successive labeling indices the rate of entry of cells into DNA-synthetic phase (S-phase) and the duration of the S-phase could be estimated. All the biopsies were classified according to the degree of gastritis. The mean (+/-SEM) length of the S-phase was found to be 7.4 +/- 0.3 hours in antral mucosa and 7.2 +/- 0.4 hours in fundic mucosa. There was no significant difference between the S-phase duration in normal mucosa, superficial gastritis, mild atrophic gastritis and severe atrophic gastritis. This observation suggests that the labeling index can be used as an expression for the rate of cell proliferation in human gastric mucosa. A significant correlation between the labelling indices and the degree of gastritis was found in both antral and fundic mucosa. In six cases, labelling indices estimated by cell counts performed on longitudinal or cross sections of foveolae were compared. Ther was no significant difference between the results obtained by the two different counting techniques.
O Hart-Hansen; A Johansen; J K Larsen; L B Svendsen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta pathologica et microbiologica Scandinavica. Section A, Pathology     Volume:  87A     ISSN:  0365-4184     ISO Abbreviation:  Acta Pathol Microbiol Scand A     Publication Date:  1979 May 
Date Detail:
Created Date:  1979-09-27     Completed Date:  1979-09-27     Revised Date:  2009-06-04    
Medline Journal Info:
Nlm Unique ID:  7508468     Medline TA:  Acta Pathol Microbiol Scand A     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  217-22     Citation Subset:  IM    
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MeSH Terms
Cell Count
Cell Division*
DNA / biosynthesis
Epithelial Cells
Gastric Mucosa / cytology*,  pathology
Gastritis / pathology*
Isotope Labeling
Middle Aged
Reg. No./Substance:
10028-17-8/Tritium; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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