Document Detail

Cell proliferation in chorionic villi at different gestational ages, as analyzed by premature chromosome condensation.
MedLine Citation:
PMID:  2265555     Owner:  NLM     Status:  MEDLINE    
The technique of premature chromosome condensation (PCC) was adapted to human first-trimester chorionic villi cells to analyze the cell-cycle kinetics of interphase chromatin. Uncultured cells of the cytotrophoblast (CT) and the mesenchymal core (MC) were obtained by a two-step digestion. PCC was induced by fusion of the chorionic interphase cells with mitotic Chinese hamster ovary or HeLa cells. Cells showing PCC in G1 (classes 1-6), S, and G2 were found. To analyze further the proliferation stages of chorionic G1 interphases, the proliferation potential index (PPI) of 34 placentae recovered between the 8th and 12th week of gestation was determined. The mean PPI found in the CT and MC cells ranged from 18% to 73%, values similar to those described for intensely proliferating tissues. The highest mean PPI value (73%) was observed in CT cells from placentae recovered at the 9th week of gestation, indicating a high specific proliferative activity of CT cells at this developmental stage.
G Raabe; K Miller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  54     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1990  
Date Detail:
Created Date:  1991-02-13     Completed Date:  1991-02-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  127-31     Citation Subset:  IM    
Department of Human Genetics, Medizinische Hochschule Hannover, FRG.
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MeSH Terms
Cell Division
Cell Line
Chorionic Villi / physiology*
Chromatin / metabolism
Chromosomes, Human / ultrastructure*
Gestational Age
Hela Cells
Mesoderm / cytology*
Pregnancy Trimester, First
Trophoblasts / cytology*
Reg. No./Substance:

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