Document Detail

Cell proliferation in the bronchial mucosa of asthmatics and chronic bronchitics.
MedLine Citation:
PMID:  7912988     Owner:  NLM     Status:  MEDLINE    
In chronic inflammatory diseases, cells are recruited but may also derive from local proliferation. In normal bronchial epithelium, under 5% of cells are in cycle but in asthma and chronic bronchitis, proliferation may occur. Cycling cells can be identified by immunohistochemistry using PC10 monoclonal antibody (Proliferating Cell Nuclear Antigen, PCNA). We enumerated PCNA-positive cells (labeling index = LI) in bronchial biopsies of 11 healthy non-smokers (HNS), seven healthy smokers (HS), 30 non-smoking asthmatics (NSA), six smoking asthmatics (SA) and 18 chronic bronchitics (CB). Twenty non-small cell lung cancer patients were used as positive control subjects. Ciliated and secretory cells were PCNA-negative. Basal cells were PCNA-positive in one of the 11 HNS (LI = 0.18 +/- 0.60), none of the seven HS, two of the 30 NSA (LI = 0.05 +/- 0.20), two of the six SA (LI = 2.4 +/- 4.3) and 11 of the 18 CB (LI = 12 +/- 20). In smokers, PCNA positivity correlated with tobacco consumption (Rho = 0.62, p < 0.0008) and in patients with chronic bronchitis, with the degree of metaplasia (tau = 0.815, p < 0.0001). The submucosa of most subjects showed no PCNA immunoreactivity. These findings suggest that the bronchial mucosa of nonsmokers is not hyperproliferative, even in asthmatics. Tobacco smoking increases PCNA immunoreactivity, possibly leading to the metaplasia of chronic bronchitis.
P Demoly; J Simony-Lafontaine; P Chanez; J L Pujol; N Lequeux; F B Michel; J Bousquet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  150     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-08-10     Completed Date:  1994-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  214-7     Citation Subset:  AIM; IM    
Clinique des Maladies Respiratoires, Hopital Arnaud de Villeneuve, Centre Hospitalier Universitaire, Montpellier, France.
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MeSH Terms
Aged, 80 and over
Asthma / pathology*
Bronchi / pathology*
Bronchitis / pathology*
Carcinoma, Non-Small-Cell Lung / pathology
Cell Division
Chronic Disease
Lung Neoplasms / pathology
Middle Aged
Mucous Membrane / pathology
Nuclear Proteins / analysis
Proliferating Cell Nuclear Antigen
Smoking / pathology
Reg. No./Substance:
0/Nuclear Proteins; 0/Proliferating Cell Nuclear Antigen

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