Document Detail

Cell proliferation of esophageal squamous epithelium in erosive and non-erosive reflux disease.
MedLine Citation:
PMID:  22110280     Owner:  NLM     Status:  MEDLINE    
AIM: To elucidate cell proliferation in erosive reflux disease (ERD) and non-erosive reflux disease (NERD), we evaluated markers in squamous epithelial cells.
METHODS: Thirty-four consecutive patients with gastroesophageal-reflux-disease-related symptoms (21 NERD and 13 ERD) were evaluated for the enrolment into the study. All patients underwent 24-h pH monitoring, standard endoscopy, and biopsy for histological evaluation. The expression of cyclins D and A was evaluated by real-time reverse transcription polymerase chain reaction (RT-PCR) from isolated epithelial cells. In all samples, analysis of the isolated cell population revealed the presence of epithelial cells only.
RESULTS: Real-time RT-PCR showed that, in patients with ERD, the relative expression of cyclin D1 mRNA in esophageal epithelium was strongly decreased in comparison with NERD patients. The mean value of relative expression of cyclin D1 mRNA in NERD patients was 3.44 ± 1.9, whereas in ERD patients, it was 1.32 ± 0.87 (P = 0.011). Real-time RT-PCR showed that, in patients with ERD, relative expression of cyclin A mRNA in esophageal epithelium was decreased in comparison with that in NERD patients (2.31 ± 2.87 vs 0.66 ± 1.11). The mean bromodeoxyuridine labeling index in the NERD patients was 5.42% ± 1.68%, whereas in ERD patients, it was 4.3% ± 1.59%.
CONCLUSION: We confirmed reduced epithelial proliferation in ERD compared with NERD patients, and that individuals who develop ERD are characterized by weaker epithelial cell proliferation.
Carlo Calabrese; Lorenzo Montanaro; Giuseppina Liguori; Elisa Brighenti; Mauela Vici; Paolo Gionchetti; Fernando Rizzello; Massimo Campieri; Massimo Derenzini; Davide Trerè
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  17     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-11-23     Completed Date:  2012-02-01     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4496-502     Citation Subset:  IM    
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MeSH Terms
Biological Markers / metabolism*
Cell Proliferation*
Cyclin A / genetics,  metabolism
Cyclin D / genetics,  metabolism
Epithelial Cells / metabolism*,  pathology,  physiology*
Esophageal pH Monitoring
Esophagus / cytology*,  pathology*
Gastroesophageal Reflux / pathology*
Reg. No./Substance:
0/Biological Markers; 0/Cyclin A; 0/Cyclin D

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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