Document Detail

Cell proliferation and cell death in periodontal ligaments during orthodontic tooth movement.
MedLine Citation:
PMID:  12009181     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to investigate cellular responses of periodontal ligaments during tooth movement. Twenty-eight male Sprague-Dawley rats, weighing 200-250 g each, were used. To create the orthodontic force, elastic rubber blocks (0.65 mm thick) were inserted between the maxillary first and second molars on both sides. On days 3, 7, 10, 14, 21 and 28 after rubber block insertion, histopathological changes in both the tension and the pressure sides were examined by immunohistochemistry using proliferating cell nuclear antigen (PCNA) and by the TUNEL method. The ratios of PCNA-positive cells on the tension side 3 and 7 days after rubber block insertion were higher than those on the pressure side. The ratios of PCNA-positive cells on the tension side were highest at day 3 after insertion and then decreased during the remainder of the experimental period. On the pressure side, the ratios of PCNA-positive cells increased up to day 10 post insertion, then decreased from 14 to 28 days. The ratios of TUNEL-positive cells on both the tension and the pressure sides increased throughout the entire experimental period. These results indicate that the periodontal ligaments on the tension side are able to respond more promptly to orthodontic forces than those on the pressure side. The data also suggest that the ratios of cell proliferation and of cell death are closely related to the regeneration and reconstruction of periodontal ligaments which reflect the orthodontic force.
R Mabuchi; K Matsuzaka; M Shimono
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of periodontal research     Volume:  37     ISSN:  0022-3484     ISO Abbreviation:  J. Periodont. Res.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-05-14     Completed Date:  2002-08-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0055107     Medline TA:  J Periodontal Res     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  118-24     Citation Subset:  D; IM    
Department of Pathology, Tokyo Dental College, Chiba, Japan.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Cell Count
Cell Death
Cell Division
Cell Nucleus / ultrastructure
Follow-Up Studies
In Situ Nick-End Labeling
Periodontal Ligament / cytology*
Proliferating Cell Nuclear Antigen / analysis
Rats, Sprague-Dawley
Regeneration / physiology
Statistics as Topic
Stress, Mechanical
Tooth Movement* / instrumentation
Tooth Root
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Proliferating Cell Nuclear Antigen; 9006-04-6/Rubber

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