Document Detail


Cell proliferation, apoptosis and mitochondrial damage in rat B50 neuronal cells after cisplatin treatment.
MedLine Citation:
PMID:  18397337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Cisplatin (cisPt) is used as a chemotherapeutic agent for the treatment of a variety of human tumours; more recently, it has been demonstrated that tumour cell exposure to cisPt ultimately results in apoptosis, but the mechanism by which nuclear cisPt/DNA generates the cytoplasmic cascade of events involved has not been clarified. We have investigated the effects of cisPt on proliferation in the neuronal cell line B50, with particular attention being given to understand whether mitochondria are a target of cisPt and their involvement in the apoptotic process. MATERIALS AND METHODS: Rat neuronal B50 cells were used to investigate the mechanisms of cisPt-induced cytotoxicity; this line has been used as a model system for neurotoxicity in vivo. RESULTS: Changes in proliferation, induction of apoptosis, activation of caspase-3 and DNA fragmentation were observed in the cells, as well as morphological and biochemical alterations of mithocondria. Activation of caspase-9 confirmed that mitochondria are a target of cisPt. CONCLUSION: CisPt exerts cytotoxic effects in the neuronal B50 cell line via a caspase-dependent pathway with mitochondria being central relay stations.
Authors:
M G Bottone; C Soldani; P Veneroni; D Avella; M Pisu; G Bernocchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-07
Journal Detail:
Title:  Cell proliferation     Volume:  41     ISSN:  1365-2184     ISO Abbreviation:  Cell Prolif.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-06-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  506-20     Citation Subset:  IM    
Affiliation:
Dipartimento di Biologia Animale, Università di Pavia, and Istituto di Genetica Moleculare, Sezione di Istochimica e Citochimica del CNR, Pavia, Italy. bottone@unipv.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Annexin A5 / metabolism
Apoptosis / drug effects*
Bromodeoxyuridine / metabolism
Caspase 9 / metabolism
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Cisplatin / pharmacology*
Cytoskeleton / drug effects,  metabolism
DNA Fragmentation / drug effects
Enzyme Activation / drug effects
Flow Cytometry
Fluorescein-5-isothiocyanate / metabolism
Immunohistochemistry
Microscopy, Confocal
Mitochondria / drug effects*,  enzymology,  pathology*
Neurons / cytology*,  drug effects*,  enzymology
Propidium / metabolism
Rats
Chemical
Reg. No./Substance:
0/Annexin A5; 15663-27-1/Cisplatin; 3326-32-7/Fluorescein-5-isothiocyanate; 36015-30-2/Propidium; 59-14-3/Bromodeoxyuridine; EC 3.4.22.-/Caspase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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