Document Detail


Cell preservation in reparative and regenerative medicine: evolution of individualized solution composition.
MedLine Citation:
PMID:  15684675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expanding complexity of biologics banked for therapeutic applications necessitates the development of improved preservation technologies for support of the emerging fields of reparative and regenerative medicine. Currently, a number of media or "solutions" are utilized for the preservation of biologics. Given the diversity of cell systems utilized in the regenerative medicine arena, we hypothesized that the development of unique (individualized) preservation solutions designed to meet the distinct molecular biological requirements of individual systems would provide for enhanced and extended preservation. To evaluate this hypothesis, coronary artery smooth muscle cells (CASMCs), coronary artery endothelial cells (CAECs), hepatic cells (C3A), and skeletal muscle cells (SKMCs) were hypothermically preserved for 2 to 7 days at 4 degrees C in either cell culture medium, University of Wisconsin Solution (UW or ViaSpan), or HypoThermosol (HTS) variants. Cells were then assayed for viability, using the alamarBlue assay as well as calcein-AM, subsequent to their return to normothermic (37 degrees C) temperatures for up to 5 days. CASMC viability was best maintained when preserved in HTS plus Trolox/EDTA, CAEC viability was highest when preserved in HTS plus Trolox, SKMCs stored in HTS plus Trolox/RGD demonstrated enhanced viability, and C3A cells were best preserved in HTS plus FK041. The data suggest that solution compositions that address the differences in cell death mechanisms limiting preservation efficacy can result in targeted improvement matched to specific cell types. These observations support the custom solution hypothesis of cell and tissue preservation.
Authors:
Aby J Mathew; John M Baust; Robert G Van Buskirk; John G Baust
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Tissue engineering     Volume:  10     ISSN:  1076-3279     ISO Abbreviation:  Tissue Eng.     Publication Date:    2004 Nov-Dec
Date Detail:
Created Date:  2005-02-01     Completed Date:  2005-05-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9505538     Medline TA:  Tissue Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1662-71     Citation Subset:  IM    
Affiliation:
Institute of Biomedical Technology, State University of New York, Binghamton, New York, USA. amathew@biolifesolutions.com
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques / methods*
Cell Survival / drug effects*
Cells, Cultured
Epithelial Cells / cytology,  drug effects,  physiology
Hepatocytes / cytology,  drug effects,  physiology
Humans
Muscle Fibers, Skeletal / cytology,  drug effects,  physiology
Myocytes, Smooth Muscle / cytology,  drug effects,  physiology
Organ Preservation Solutions / chemistry*,  classification,  pharmacology*
Regeneration / drug effects,  physiology
Tissue Engineering / methods*
Tissue Preservation / methods*
Grant Support
ID/Acronym/Agency:
R44 HL64-999-01/HL/NHLBI NIH HHS; R44 RR14185/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Organ Preservation Solutions

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