Document Detail


Cell population kinetics of the mouse lens epithelium.
MedLine Citation:
PMID:  7251687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The dividing lens epithelium of 8-week-old CF1 mice consists of a monocellular layer of about 31,000 cells and does not include the postmitotic cells of the meridional rows and another postmitotic zone of seven cell positions' width immediately anterior to the rows. The latter two populations contain approximately 3,600 and 9,000 cells, respectively, for a total of 44,000 cells in the entire lens epithelium. Autoradiographic analysis based upon mitotic index and cell cycle times indicates that the epithelium produces 207 new lens fibers a day. Throughout the 20-day period of study, labeled cells appeared almost entirely as pairs following a single dose of 3H-thymidine and clusters of labeled nuclei were not seen. Moreover, the number of labeled cells dropped only slowly with time, as did the grain counts. These observations indicate that logarithmic division "cascade" does not occur in the lens. The dividing cell population consists largely of a slowly cycling stem cell group, dividing once about every 17-20 days, and consisting of some 5,000 cells. A subpopulation may exist which undergoes two rapid consecutive divisions before becoming postmitotic, but this is too small to make a significant contribution to lens fiber production. Four days are required to transit the postmitotic zone, and an additional 43 or so are needed to transit the meridional rows and differentiate into anucleate lens fibers. Data from other laboratories indicate that the entire process, from mitosis to final differentiation, requires about 4 months. Hence, most of this time is spent in migration of nondividing cells.
Authors:
N S Rafferty; K A Rafferty
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  107     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1981 Jun 
Date Detail:
Created Date:  1981-09-22     Completed Date:  1981-09-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  309-15     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Carbon Radioisotopes
Cell Count
Epithelial Cells
Kinetics
Lens, Crystalline / anatomy & histology*,  metabolism
Male
Mice
Thymidine / metabolism
Time Factors
Tritium
Grant Support
ID/Acronym/Agency:
CA 13494/CA/NCI NIH HHS; EY 00698/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 10028-17-8/Tritium; 50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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