Document Detail


Cell partitioning in aqueous two-phase polymer systems.
MedLine Citation:
PMID:  17924080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This review addresses whole cell separation and isolation using aqueous two-phase systems based on biocompatible polymers. The physicochemical factors that influence phase separation and systems properties are analysed. Especially, emphasis is given to the polyethylene glycol (PEG) and dextran two-phase systems and to stimuli-responsive soluble-insoluble polymers. The major factors that affect cell partitioning, such as polymer molecular weight and concentration, temperature, ionic species and pH, and affinity extraction, are also evaluated taking into account the cell types and cell surface properties. The applications of aqueous two-phase separation in cell processing are described, namely the new developments in continuous cell partitioning in microdevices and extractive bioconversions with relevance to the biomedical sector.
Authors:
J M S Cabral
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Advances in biochemical engineering/biotechnology     Volume:  106     ISSN:  0724-6145     ISO Abbreviation:  Adv. Biochem. Eng. Biotechnol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-10-09     Completed Date:  2008-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307733     Medline TA:  Adv Biochem Eng Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  151-71     Citation Subset:  IM    
Affiliation:
Institute for Biotechnology and Bioengineering, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. joaquim.cabral@ist.utl.pt
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods*
Humans
Polymers / chemistry*
Water / chemistry
Chemical
Reg. No./Substance:
0/Polymers; 7732-18-5/Water

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