| Cell number and timing of transplantation determine survival of human neural stem cell grafts in stroke-damaged rat brain. | |
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MedLine Citation:
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PMID: 20531461 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neural stem cells (NSCs) derived from human fetal striatum and transplanted as neurospheres survive in stroke-damaged striatum, migrate from the implantation site, and differentiate into mature neurons. Here, we investigated how various steps of neurogenesis are affected by intrastriatal transplantation of human NSCs at different time points after stroke and with different numbers of cells in each implant. Rats were subjected to middle cerebral artery occlusion and then received intrastriatal transplants of NSCs. Transplantation shortly after stroke (48 hours) resulted in better cell survival than did transplantation 6 weeks after stroke, but the delayed transplantation did not influence the magnitude of migration, neuronal differentiation, and cell proliferation in the grafts. Transplanting greater numbers of grafted NSCs did not result in a greater number of surviving cells or increased neuronal differentiation. A substantial number of activated microglia was observed at 48 hours after the insult in the injured striatum, but reached maximum levels 1 to 6 weeks after stroke. Our findings show that the best survival of grafted human NSCs in stroke-damaged brain requires optimum numbers of cells to be transplanted in the early poststroke phase, before the inflammatory response is established. These findings, therefore, have direct clinical implications. |
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Authors:
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Vladimer Darsalia; Susan J Allison; Carlo Cusulin; Emanuela Monni; Daniela Kuzdas; Therése Kallur; Olle Lindvall; Zaal Kokaia |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-09 |
Journal Detail:
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Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 31 ISSN: 1559-7016 ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-04 Completed Date: 2011-02-03 Revised Date: 2012-01-02 |
Medline Journal Info:
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Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: United States |
Other Details:
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Languages: eng Pagination: 235-42 Citation Subset: IM |
Affiliation:
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Laboratory of Neural Stem Cell Biology and Therapy, University Hospital, Lund, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / pathology* Cell Count Cell Differentiation / physiology Cell Proliferation Embryonic Stem Cells Graft Survival Humans Immunohistochemistry Infarction, Middle Cerebral Artery / pathology, therapy Male Microglia / physiology Neural Stem Cells / transplantation* Neurogenesis / physiology Rats Rats, Wistar Stroke / pathology, surgery* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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