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Cell nucleus and DNA fragmentation are not required for apoptosis.
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MedLine Citation:
PMID:  7523418     Owner:  NLM     Status:  MEDLINE    
Apoptosis is the predominant form of cell death and occurs under a variety of physiological and pathological conditions. Cells undergoing apoptotic cell death reveal a characteristic sequence of cytological alterations including membrane blebbing and nuclear and cytoplasmic condensation. Activation of an endonuclease which cleaves genomic DNA into internucleosomal DNA fragments is considered to be the hallmark of apoptosis. However, no clear evidence exists that DNA degradation plays a primary and causative role in apoptotic cell death. Here we show that cells enucleated with cytochalasin B still undergo apoptosis induced either by treatment with menadione, an oxidant quinone compound, or by triggering APO-1/Fas, a cell surface molecule involved in physiological cell death. Incubation of enucleated cells with the agonistic monoclonal anti-APO-1 antibody revealed the key morphological features of apoptosis. Moreover, in non-enucleated cells inhibitors of endonuclease blocked DNA fragmentation, but not cell death induced by anti-APO-1. These data suggest that DNA degradation and nuclear signaling are not required for induction of apoptotic cell death.
K Schulze-Osthoff; H Walczak; W Dröge; P H Krammer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  127     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-10-31     Completed Date:  1994-10-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  15-20     Citation Subset:  IM    
Division of Immunochemistry, Deutsches Krebsforschungszentrum, Heidelberg, FRG.
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MeSH Terms
Antibodies, Monoclonal
Antigens, CD95
Antigens, Surface / physiology
Apoptosis / drug effects,  physiology*
Aurintricarboxylic Acid / pharmacology
Cell Membrane / drug effects
Cell Nucleus / drug effects,  physiology*
Cell Survival
DNA / metabolism*
Deoxyribonuclease I / antagonists & inhibitors
L Cells (Cell Line)
Vitamin K / pharmacology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD95; 0/Antigens, Surface; 12001-79-5/Vitamin K; 4431-00-9/Aurintricarboxylic Acid; 9007-49-2/DNA; EC I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 10 Year: 1994
Volume: 127 Issue: 1
First Page: 15 Last Page: 20
ID: 2120176
Publisher Id: 95014705
PubMed Id: 7523418

Cell nucleus and DNA fragmentation are not required for apoptosis

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