Document Detail

Cell nanomechanics and focal adhesions are regulated by retinol and conjugated linoleic acid in a dose-dependent manner.
MedLine Citation:
PMID:  19546491     Owner:  NLM     Status:  MEDLINE    
Retinol and conjugated linoleic acid (CLA) have previously been shown to have an important role in gene expression and various cellular processes, including differentiation, proliferation and cell death. In this study we have investigated the effect of retinol and CLA, both individually and in combination, on the intracellular cytoskeleton, focal adhesions (FAs) and the nanomechanical properties of 3T3 fibroblasts. We observed a dose-dependent decrease in the formation of FAs following treatment with either compound, which was directly correlated to an increase in cell height (>30%) and a decrease in the measured Young's modulus (approximately 28%). Furthermore, treatments with both compounds demonstrated an increased effect and led to a reduction of >70% in the average number of FAs per cell and a decrease of >50% in average cell stiffness. These data reveal that retinol and CLA disrupt FA formation, leading to an increase in cell height and a significant decrease in stiffness. These results may broaden our understanding of the interplay between cell nanomechanics and cellular contact with the external microenvironment, and help to shed light on the important role of retinoids and CLA in health and disease.
Yaron R Silberberg; Gleb E Yakubov; Michael A Horton; Andrew E Pelling
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-23
Journal Detail:
Title:  Nanotechnology     Volume:  20     ISSN:  1361-6528     ISO Abbreviation:  Nanotechnology     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-24     Completed Date:  2009-08-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101241272     Medline TA:  Nanotechnology     Country:  England    
Other Details:
Languages:  eng     Pagination:  285103     Citation Subset:  IM    
The London Centre for Nanotechnology and Centre for NanoMedicine, University College London, London, UK.
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MeSH Terms
3T3 Cells
Biomechanics / drug effects*
Cell Shape / drug effects
Cytoskeleton / drug effects,  metabolism
Dose-Response Relationship, Drug
Focal Adhesions / metabolism*
Linoleic Acids, Conjugated / pharmacology*
Microscopy, Atomic Force
Microscopy, Confocal
Vitamin A / pharmacology*
Vitamins / pharmacology*
Grant Support
//Biotechnology and Biological Sciences Research Council; //Wellcome Trust
Reg. No./Substance:
0/Linoleic Acids, Conjugated; 0/Vitamins; 11103-57-4/Vitamin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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