Document Detail

Cell motion model for moving dielectrophoresis.
MedLine Citation:
PMID:  18558772     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Moving dielectrophoresis has been recently developed by the authors as an alternative method to achieve simultaneous cell fractionation and transportation. With an array of independently excitable microelectrodes, this method generates a moving electric field to sequentially fractionate and transport cells across a microchannel. Due to the peculiarity of this method, the motion of the cells is unsteady and there are interesting and distinct differences between cells experiencing positive or negative dielectrophoresis. For a proper understanding and design of a microdevice utilizing this methodology, this study presents a model for the equation of motion for a polarized cell and its unsteady motion under moving dielectrophoresis. The model considers the basic module to generate a moving electric field, where there is a finite-width top electrode and an infinite-width bottom electrode, in a parallel-plate configuration. The forces considered include dielectrophoretic force, fluid drag, buoyancy, and gravitational force. These forces are modeled as equivalent point forces acting at the center of mass of the cell. A parallel-plate wall correction factor is employed to account for the effect of the large cell size to microchannel height ratio. Various parameters are examined including the initial position of the cell relative to the electrodes, cell's Clausius-Mossotti factor, cell size, applied voltage, electrode width, interelectrode gap, microchannel height, number of energized electrodes, and types of electrode configurations. Reasonable agreements were obtained between simulated and experimental results. As the solution of the unsteady motion is rather tedious, a MATLAB algorithm, with all the associated files, for the prediction of the cell trajectory, is available on request.
Chin Hock Kua; Yee Cheong Lam; Isabel Rodriguez; Chun Yang; Kamal Youcef-Toumi
Publication Detail:
Type:  Journal Article     Date:  2008-06-18
Journal Detail:
Title:  Analytical chemistry     Volume:  80     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-14     Completed Date:  2008-08-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5454-61     Citation Subset:  -    
Singapore-MIT Alliance, and School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798.
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