Document Detail

Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy.
MedLine Citation:
PMID:  20306502     Owner:  NLM     Status:  MEDLINE    
The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell parameters including absolute volume, shape and integral intracellular refractive index. In combination with the numerous different specific fluorescent cellular probes, this multimodality microscopy can address important issues in cell biology. This is demonstrated by the study of intracellular calcium homeostasis associated with the change in cell volume, which play a critical role in the excitotoxicity-induced neuronal death.
Nicolas Pavillon; Alexander Benke; Daniel Boss; Corinne Moratal; Jonas Kühn; Pascal Jourdain; Christian Depeursinge; Pierre J Magistretti; Pierre Marquet
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biophotonics     Volume:  3     ISSN:  1864-0648     ISO Abbreviation:  J Biophotonics     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-10-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101318567     Medline TA:  J Biophotonics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  432-6     Citation Subset:  IM    
Copyright Information:
(c) 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Advanced Photonics Laboratory (MVD), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aniline Compounds
Calcium / metabolism*
Cell Death
Cell Size
Cells, Cultured
Cerebral Cortex / cytology,  metabolism
Glutamic Acid / metabolism
Holography / instrumentation,  methods*
Intracellular Space / metabolism*
Ions / metabolism
Microscopy, Fluorescence / instrumentation,  methods*
Neurons / cytology*,  metabolism*,  pathology
Time Factors
Reg. No./Substance:
0/Aniline Compounds; 0/Fluo 4; 0/Ions; 0/Xanthenes; 56-86-0/Glutamic Acid; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Psychobiological models of adolescent risk: implications for prevention and intervention.
Next Document:  Synthesis, Magnetic Properties, and STM spectroscopy of Cobalt(II) Cubanes [Co(II) (4)(Cl)(4)(HL)(4)...