| Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy. | |
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MedLine Citation:
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PMID: 20306502 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell parameters including absolute volume, shape and integral intracellular refractive index. In combination with the numerous different specific fluorescent cellular probes, this multimodality microscopy can address important issues in cell biology. This is demonstrated by the study of intracellular calcium homeostasis associated with the change in cell volume, which play a critical role in the excitotoxicity-induced neuronal death. |
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Authors:
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Nicolas Pavillon; Alexander Benke; Daniel Boss; Corinne Moratal; Jonas Kühn; Pascal Jourdain; Christian Depeursinge; Pierre J Magistretti; Pierre Marquet |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biophotonics Volume: 3 ISSN: 1864-0648 ISO Abbreviation: J Biophotonics Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-23 Completed Date: 2010-10-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101318567 Medline TA: J Biophotonics Country: Germany |
Other Details:
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Languages: eng Pagination: 432-6 Citation Subset: IM |
Copyright Information:
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(c) 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Advanced Photonics Laboratory (MVD), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. nicolas.pavillon@a3.epfl.ch |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aniline Compounds Animals Calcium / metabolism* Cell Death Cell Size Cells, Cultured Cerebral Cortex / cytology, metabolism Fluorescence Glutamic Acid / metabolism Holography / instrumentation, methods* Homeostasis Intracellular Space / metabolism* Ions / metabolism Mice Microscopy, Fluorescence / instrumentation, methods* Neurons / cytology*, metabolism*, pathology Time Factors Xanthenes |
| Chemical | |
Reg. No./Substance:
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0/Aniline Compounds; 0/Fluo 4; 0/Ions; 0/Xanthenes; 56-86-0/Glutamic Acid; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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