Document Detail


Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy.
MedLine Citation:
PMID:  20306502     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell parameters including absolute volume, shape and integral intracellular refractive index. In combination with the numerous different specific fluorescent cellular probes, this multimodality microscopy can address important issues in cell biology. This is demonstrated by the study of intracellular calcium homeostasis associated with the change in cell volume, which play a critical role in the excitotoxicity-induced neuronal death.
Authors:
Nicolas Pavillon; Alexander Benke; Daniel Boss; Corinne Moratal; Jonas Kühn; Pascal Jourdain; Christian Depeursinge; Pierre J Magistretti; Pierre Marquet
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biophotonics     Volume:  3     ISSN:  1864-0648     ISO Abbreviation:  J Biophotonics     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-10-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101318567     Medline TA:  J Biophotonics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  432-6     Citation Subset:  IM    
Copyright Information:
(c) 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Advanced Photonics Laboratory (MVD), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. nicolas.pavillon@a3.epfl.ch
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MeSH Terms
Descriptor/Qualifier:
Aniline Compounds
Animals
Calcium / metabolism*
Cell Death
Cell Size
Cells, Cultured
Cerebral Cortex / cytology,  metabolism
Fluorescence
Glutamic Acid / metabolism
Holography / instrumentation,  methods*
Homeostasis
Intracellular Space / metabolism*
Ions / metabolism
Mice
Microscopy, Fluorescence / instrumentation,  methods*
Neurons / cytology*,  metabolism*,  pathology
Time Factors
Xanthenes
Chemical
Reg. No./Substance:
0/Aniline Compounds; 0/Fluo 4; 0/Ions; 0/Xanthenes; 56-86-0/Glutamic Acid; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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