Document Detail

Cell morphological response to low shear stress in a two-dimensional culture microsystem with magnitudes comparable to interstitial shear stress.
MedLine Citation:
PMID:  21084742     Owner:  NLM     Status:  In-Process    
Slow interstitial flow can lead to large changes in cell morphology. Since conventional biological assays are adapted to two-dimensional culture protocols, there is a need to develop a microfluidic system that can generate physiological levels of interstitial flow. Here we developed a system that uses a passive osmotic pumping mechanism to generate sustained and steady interstitial flows for two-dimensional cultures. Two different cell types, fibroblasts and mesenchymal stem cells, were selected because they are generally exposed to interstitial flow. To quantify the cellular response to interstitial shear flow in terms of proliferation and alignment, 4 rates of flow were applied. We found that the proliferation rate of fibroblasts varied linearly with wall shear stress. In addition, alignment of fibroblast cells depended linearly on the magnitude of the shear stress, whereas mesenchymal stem cells were aligned regardless of the magnitude of shear stress. This suggested that mesenchymal stem cells are very sensitive to shear stresses, even at levels generated by interstitial flow. The results presented here emphasize the need to consider the mechanosensitivity and the natural role of different cell types when evaluating their responses to fluid flow.
Joong Yull Park; Sung Ju Yoo; Lalit Patel; Soon Hyuck Lee; Sang-Hoon Lee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biorheology     Volume:  47     ISSN:  1878-5034     ISO Abbreviation:  Biorheology     Publication Date:  2010  
Date Detail:
Created Date:  2010-11-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372526     Medline TA:  Biorheology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  165-78     Citation Subset:  IM    
Department of Biomedical Engineering, College of Health Science, Korea University, Seoul, Korea.
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