| Cell and molecular anatomy of nicotinic acetylcholine receptor subunits and calcitonin gene-related peptide in the rat vestibular system. | |
| | |
MedLine Citation:
|
PMID: 1762788 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this report we demonstrate the pattern of calcitonin gene-related peptide (CGRP) mRNA and immunoreactivity in the central and peripheral vestibular system of the rat, using a CGRP cRNA probe and a polyclonal CGRP antiserum. We present evidence that somata in all regions of efferent vestibular neurons contain CGRP based on the correspondence between in situ hybridization (mRNA) and immunohistochemistry (mRNA translation product). CGRP immunohistochemistry (CGRPi) and in situ hybridization confirm that CGRPi axons and terminals present in the vestibular neuroepithelium are efferent in origin. Immunoelectron microscopy revealed an extensive innervation of the afferent vestibular pathway by CGRPi terminals that was not limited to the primary afferent chalice, as previously reported by Tanaka et al. (Brain Res 1989;504:31-5). An efferent neuromodulatory role of CGRP can be inferred from the distribution of terminals found on the primary afferent fibers, and type I and type II hair cells. In addition, we present evidence that nicotinic acetylcholine receptor (nAChR) subunit mRNA is expressed by primary afferent cell bodies. On the basis of these data, a hypothetical molecular mechanism of vestibular efferent modulation of the primary afferent pathway is proposed. |
| | |
Authors:
|
P A Wackym; P Popper; P H Ward; P E Micevych |
Related Documents
:
|
11793338 - Developmental expression of monoamine oxidases a and b in the central and peripheral ne... 10448218 - Generation of cerebellar granule neurons in vivo by transplantation of bmp-treated neur... 1359468 - Lesions of nigrostriatal pathway reduce expression of tyrosine hydroxylase gene in resi... 2472998 - Expression of substance p/neurokinin a-encoding preprotachykinin messenger ribonucleic ... 2164948 - Effects of long-term diabetes and galactosaemia upon lens and retinal mrna levels in th... 14998908 - Differential expression of the vascular endothelial growth factor-receptor system in th... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery Volume: 105 ISSN: 0194-5998 ISO Abbreviation: Otolaryngol Head Neck Surg Publication Date: 1991 Oct |
Date Detail:
|
Created Date: 1992-02-11 Completed Date: 1992-02-11 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8508176 Medline TA: Otolaryngol Head Neck Surg Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 493-510 Citation Subset: IM; S |
Affiliation:
|
Victor Goodhill Ear Center, Division of Head and Neck Surgery, UCLA School of Medicine 90024-1624. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Calcitonin Gene-Related Peptide / analysis*, immunology Immunohistochemistry Male Microscopy, Immunoelectron Models, Molecular Neurons, Afferent / cytology, ultrastructure Neurons, Efferent / cytology RNA Probes RNA, Messenger / analysis Rats Rats, Inbred Strains Receptors, Nicotinic / analysis*, ultrastructure Vestibule, Labyrinth / anatomy & histology, chemistry*, innervation, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
|
K08DC00062/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/RNA Probes; 0/RNA, Messenger; 0/Receptors, Nicotinic; 83652-28-2/Calcitonin Gene-Related Peptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Experimental nerve regeneration. A review.
Next Document: The protective effects of tirilated mesylate (U74006F) on ischemic and reperfusion-induced cochlear ...