Document Detail


Cell and molecular anatomy of nicotinic acetylcholine receptor subunits and calcitonin gene-related peptide in the rat vestibular system.
MedLine Citation:
PMID:  1762788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this report we demonstrate the pattern of calcitonin gene-related peptide (CGRP) mRNA and immunoreactivity in the central and peripheral vestibular system of the rat, using a CGRP cRNA probe and a polyclonal CGRP antiserum. We present evidence that somata in all regions of efferent vestibular neurons contain CGRP based on the correspondence between in situ hybridization (mRNA) and immunohistochemistry (mRNA translation product). CGRP immunohistochemistry (CGRPi) and in situ hybridization confirm that CGRPi axons and terminals present in the vestibular neuroepithelium are efferent in origin. Immunoelectron microscopy revealed an extensive innervation of the afferent vestibular pathway by CGRPi terminals that was not limited to the primary afferent chalice, as previously reported by Tanaka et al. (Brain Res 1989;504:31-5). An efferent neuromodulatory role of CGRP can be inferred from the distribution of terminals found on the primary afferent fibers, and type I and type II hair cells. In addition, we present evidence that nicotinic acetylcholine receptor (nAChR) subunit mRNA is expressed by primary afferent cell bodies. On the basis of these data, a hypothetical molecular mechanism of vestibular efferent modulation of the primary afferent pathway is proposed.
Authors:
P A Wackym; P Popper; P H Ward; P E Micevych
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery     Volume:  105     ISSN:  0194-5998     ISO Abbreviation:  Otolaryngol Head Neck Surg     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1992-02-11     Completed Date:  1992-02-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8508176     Medline TA:  Otolaryngol Head Neck Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  493-510     Citation Subset:  IM; S    
Affiliation:
Victor Goodhill Ear Center, Division of Head and Neck Surgery, UCLA School of Medicine 90024-1624.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcitonin Gene-Related Peptide / analysis*,  immunology
Immunohistochemistry
Male
Microscopy, Immunoelectron
Models, Molecular
Neurons, Afferent / cytology,  ultrastructure
Neurons, Efferent / cytology
RNA Probes
RNA, Messenger / analysis
Rats
Rats, Inbred Strains
Receptors, Nicotinic / analysis*,  ultrastructure
Vestibule, Labyrinth / anatomy & histology,  chemistry*,  innervation,  ultrastructure
Grant Support
ID/Acronym/Agency:
K08DC00062/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/RNA Probes; 0/RNA, Messenger; 0/Receptors, Nicotinic; 83652-28-2/Calcitonin Gene-Related Peptide

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