| Cell migration analyses within fibroblast-derived 3-D matrices. | |
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MedLine Citation:
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PMID: 15576907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Research in cell biology often is based in tissue culturing cells on artificial substrates, such as plastic or glass. These artificial conditions are prone to distorting findings by persuading cells to adjust to artificial flat rigid surfaces. In contrast, the natural substrate for most cells in living organisms is the extracellular matrix (ECM), which is three-dimensional, complex, and dynamic in its molecular composition, and variable in pliability. Here we show how to measure the rates and directionality of fibroblasts motility within physiologically relevant in vivo-like cell-derived three-dimensional matrices. |
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Authors:
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Edna Cukierman |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 294 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2005 |
Date Detail:
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Created Date: 2004-12-03 Completed Date: 2005-04-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 79-93 Citation Subset: IM |
Affiliation:
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Tumor Cell Biology, Basic Science, Fox Chase Cancer Center, Philadelphia, PA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Cell Culture Techniques / methods Cell Movement / physiology* Culture Media Extracellular Matrix / physiology*, ultrastructure Fibroblasts / cytology*, physiology* Indicators and Reagents Mice |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Indicators and Reagents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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