Document Detail


Cell microarray for screening feeder cells for differentiation of embryonic stem cells.
MedLine Citation:
PMID:  16243279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microarrays are currently recognized as one of major tools in the assessment of gene expression via cDNA or RNA analysis and are now accepted as a powerful experimental tool for high-throughput screening of a large number of samples, such as cDNA and siRNAs. In this study, we examined the potential of the microarray methodology for high-throughput screening of candidate cells as feeder cells which effectively differentiate embryonic stem (ES) cells to the specific lineage. Cell arrays were prepared by applying three kinds of cells, PA6, human umbilical vein endothelial, and COS-1 cells, to circular spots, 2 mm in diameter, on a glass plate, followed by the application of mouse ES cells to the cell microarray. After 8 d in culture, TuJ1 (neuron-specific class III beta-tubulin) immunocytochemical staining clearly demonstrated that only PA6 cell spots had the capability to induce ES cells to neuronal differentiation. Although this is a model experiment, these findings clearly indicate that the cell microarray will become a powerful tool for high-throughput screening large numbers of candidate feeder cells for specific differentiation.
Authors:
Hironori Yamazoe; Hiroo Iwata
Related Documents :
6757259 - A clonal analysis of the differentiation of 3t3-l1 preadipose cells: role of insulin.
18463099 - Lysosome dispersion in osteoblasts accommodates enhanced collagen production during dif...
21440059 - A study of the relative importance of peroxiredoxin, catalase and glutathione-dependent...
3163939 - Modulation of 6-thioguanine activity by guanine in human promyelocytic leukemia hl-60 c...
16107889 - Translational study of vitamin d differentiation therapy of myeloid leukemia: effects o...
3089289 - Appearance of the arachidonic acid metabolic pathway in human promyelocytic leukemia (h...
9466669 - Overexpression of p21waf1/cip1 induces cell differentiation and growth inhibition in a ...
19383399 - Isolation of major pancreatic cell types and long-term culture-initiating cells using n...
21558269 - Plasma membrane aquaporin aqpz protein is essential for glucose metabolism during photo...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  100     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-10-24     Completed Date:  2005-11-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  292-6     Citation Subset:  IM    
Affiliation:
Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biotechnology / methods*
COS Cells
Cell Differentiation / genetics
Cercopithecus aethiops
Coculture Techniques
Embryo, Mammalian / cytology*
Humans
Mice
Neurons / cytology
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / cytology*,  physiology
Tissue Array Analysis*
Umbilical Veins / cytology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A new microbial method for more efficient production of Nalpha-benzyloxycarbonyl-L-aminoadipate delt...
Next Document:  Performance of a styrene-degrading biofilter inoculated with Pseudomonas sp. SR-5.