Document Detail

Cell microarray for screening feeder cells for differentiation of embryonic stem cells.
MedLine Citation:
PMID:  16243279     Owner:  NLM     Status:  MEDLINE    
Microarrays are currently recognized as one of major tools in the assessment of gene expression via cDNA or RNA analysis and are now accepted as a powerful experimental tool for high-throughput screening of a large number of samples, such as cDNA and siRNAs. In this study, we examined the potential of the microarray methodology for high-throughput screening of candidate cells as feeder cells which effectively differentiate embryonic stem (ES) cells to the specific lineage. Cell arrays were prepared by applying three kinds of cells, PA6, human umbilical vein endothelial, and COS-1 cells, to circular spots, 2 mm in diameter, on a glass plate, followed by the application of mouse ES cells to the cell microarray. After 8 d in culture, TuJ1 (neuron-specific class III beta-tubulin) immunocytochemical staining clearly demonstrated that only PA6 cell spots had the capability to induce ES cells to neuronal differentiation. Although this is a model experiment, these findings clearly indicate that the cell microarray will become a powerful tool for high-throughput screening large numbers of candidate feeder cells for specific differentiation.
Hironori Yamazoe; Hiroo Iwata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  100     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-10-24     Completed Date:  2005-11-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  292-6     Citation Subset:  IM    
Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
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MeSH Terms
Biotechnology / methods*
COS Cells
Cell Differentiation / genetics
Cercopithecus aethiops
Coculture Techniques
Embryo, Mammalian / cytology*
Neurons / cytology
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / cytology*,  physiology
Tissue Array Analysis*
Umbilical Veins / cytology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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