Document Detail

Cell membrane-derived lysophosphatidylcholine activates cardiac ryanodine receptor channels.
MedLine Citation:
PMID:  17028837     Owner:  NLM     Status:  MEDLINE    
Lysophosphatidylcholine (LPC) is metabolized from a membrane phospholipid and modulates a variety of channels in the plasma membrane (PM). We examined LPC modulation of cardiac ryanodine receptor (RyR) channels in the sarcoplasmic reticulum (SR) using the planar lipid bilayer method to measure the single-channel currents. Micromolar concentrations of LPC increased the open probability of the reconstituted RyR channels irrespective of whether LPC was added to the cis or trans chamber. LPC also increased the membrane capacitance of the bilayer. The effects of LPC contrasted well with those of sphingosylphosphorylcholine (SPC). Taken together, these results suggest that amphipathic lipid LPC does not bind directly to the RyR channel protein, but rather, is incorporated into the bilayer membrane and activates the channel. Thus, we consider cell membrane-derived LPC to be a putative endogenous mediator that activates not only plasma membrane channels but also RyR channels and induces arrhythmogenic Ca(2+) mobilization in cardiomyocytes.
Yuki Nakamura; Midori Yasukochi; Sei Kobayashi; Kiyoko Uehara; Akira Honda; Ryuji Inoue; Issei Imanaga; Akira Uehara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-07
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  453     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-20     Completed Date:  2007-06-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  455-62     Citation Subset:  IM    
Department of Physiology, School of Medicine, Fukuoka University, 45-1, 7-chome Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan.
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MeSH Terms
Biological Transport / physiology
Calcium / metabolism
Heart / drug effects
Intracellular Membranes / chemistry*,  metabolism,  physiology
Lipid Bilayers / chemistry,  metabolism
Lysophosphatidylcholines / pharmacology*
Membrane Potentials / drug effects
Models, Biological
Myocardium / metabolism*
Ryanodine Receptor Calcium Release Channel / metabolism,  physiology*
Sarcoplasmic Reticulum / drug effects,  metabolism
Reg. No./Substance:
0/Lipid Bilayers; 0/Lysophosphatidylcholines; 0/Ryanodine Receptor Calcium Release Channel; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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