| Cell membrane-derived lysophosphatidylcholine activates cardiac ryanodine receptor channels. | |
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MedLine Citation:
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PMID: 17028837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lysophosphatidylcholine (LPC) is metabolized from a membrane phospholipid and modulates a variety of channels in the plasma membrane (PM). We examined LPC modulation of cardiac ryanodine receptor (RyR) channels in the sarcoplasmic reticulum (SR) using the planar lipid bilayer method to measure the single-channel currents. Micromolar concentrations of LPC increased the open probability of the reconstituted RyR channels irrespective of whether LPC was added to the cis or trans chamber. LPC also increased the membrane capacitance of the bilayer. The effects of LPC contrasted well with those of sphingosylphosphorylcholine (SPC). Taken together, these results suggest that amphipathic lipid LPC does not bind directly to the RyR channel protein, but rather, is incorporated into the bilayer membrane and activates the channel. Thus, we consider cell membrane-derived LPC to be a putative endogenous mediator that activates not only plasma membrane channels but also RyR channels and induces arrhythmogenic Ca(2+) mobilization in cardiomyocytes. |
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Authors:
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Yuki Nakamura; Midori Yasukochi; Sei Kobayashi; Kiyoko Uehara; Akira Honda; Ryuji Inoue; Issei Imanaga; Akira Uehara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-07 |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 453 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-20 Completed Date: 2007-06-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: Germany |
Other Details:
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Languages: eng Pagination: 455-62 Citation Subset: IM |
Affiliation:
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Department of Physiology, School of Medicine, Fukuoka University, 45-1, 7-chome Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan. Ueharaak@fukuoka-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport / physiology Calcium / metabolism Heart / drug effects Intracellular Membranes / chemistry*, metabolism, physiology Lipid Bilayers / chemistry, metabolism Lysophosphatidylcholines / pharmacology* Membrane Potentials / drug effects Models, Biological Myocardium / metabolism* Rabbits Ryanodine Receptor Calcium Release Channel / metabolism, physiology* Sarcoplasmic Reticulum / drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Lipid Bilayers; 0/Lysophosphatidylcholines; 0/Ryanodine Receptor Calcium Release Channel; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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